The 3'-untranslated region of the alpha2C-adrenergic receptor mRNA impedes translation of the receptor message.

The Journal of Biological Chemistry
Q YangS M Lanier

Abstract

We report that two subtypes of alpha2-adrenergic receptors (alpha2A/D- and alpha2C-AR) are ectopically expressed with dramatically different efficiencies and that this difference is due to a 288-nucleotide (nt) segment in the 3'-untranslated region (3'-UTR) of the alpha2C-AR mRNA that impairs translational processing. NIH-3T3 fibroblasts were transfected with receptor constructs (coding region plus 552 nt, alpha2C-AR; coding region plus 1140 nt, alpha2A/D-AR) and a vector conferring G418 resistance. Transcription was driven by the murine sarcoma virus promoter element, and the receptor gene segment was upstream of an SV40 polyadenylation cassette. Drug-resistant transfectants were evaluated for expression of receptor mRNA and protein. 90% of the NIH-3T3 alpha2C-AR transfectants expressed receptor mRNA, but only 14% of the clonal cell lines expressed receptor protein. In contrast, 90% of the NIH-3T3 alpha2A/D-AR transfectants expressed receptor protein (200-5000 fmol/mg). Similar results were obtained following transfection of DDT1MF-2 cells with the two receptor constructs. The role of the 3'-UTR of the alpha2C-AR in mRNA processing was determined by generating new constructs in which the 3'-UTR was progressively truncated from...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·M Kozak
May 26, 1994·Nature·E R Gavis, R Lehmann
Jun 30, 1995·Journal of Molecular Biology·A P GultyaevC W Pleij
Jun 7, 1995·Journal of Theoretical Biology·F H van BatenburgC W Pleij
Sep 1, 1995·Microbiological Reviews·J Ross
Apr 1, 1994·Current Opinion in Genetics & Development·N Sonenberg
Jan 1, 1994·Brain Research. Molecular Brain Research·M ScheininR T Fremeau
Feb 22, 1993·The Journal of Comparative Neurology·A P NicholasT Hökfelt
Mar 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Sommerville, M Ladomery
Jan 1, 1996·Annual Review of Biochemistry·A Jacobson, S W Peltz
Aug 12, 1996·The Journal of Comparative Neurology·E M TalleyK R Lynch
Aug 12, 1996·The Journal of Comparative Neurology·D L RosinK R Lynch
Oct 1, 1996·The International Journal of Biochemistry & Cell Biology·R HovlandI F Pryme

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Citations

Oct 20, 1999·Journal of Molecular Neuroscience : MN·S AronovI Ginzburg
Dec 5, 1998·Brain Research. Molecular Brain Research·B Kavety, J I Morgan
Aug 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kersten M SmallStephen B Liggett
Aug 22, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qifang WuHorace H Loh
Feb 27, 2007·Biochimica Et Biophysica Acta·Natalia BunimovOdette Laneuville
Apr 4, 2006·Biochemical and Biophysical Research Communications·Ashleigh SartorMaria A Burnatowska-Hledin
Nov 13, 2004·The Journal of Biological Chemistry·Karthikeyan KandasamyBaby G Tholanikunnel
Apr 27, 2004·The Journal of Biological Chemistry·Kothandharaman SubramaniamBaby G Tholanikunnel
Nov 20, 1998·Molecular and Cellular Biology·A SpicherH M Blau
Nov 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jae-Kwan KimWon-Ha Lee

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