The 3T3-L1 fibroblast to adipocyte conversion is accompanied by increased expression of angiopoietin-1, a ligand for tie2

Growth Factors
S A StackerA F Wilks

Abstract

The tie2 receptor tyrosine kinase plays a key role in angiogenesis, and the remodeling and maturation of blood vessels. In this study we have used a factor-dependent cell line (Ba/F3) expressing a chimeric receptor containing the extracellular domain of mouse tie2 and the transmembrane and cytoplasmic domain of the erythropoietin receptor to identify specific binding activity associated with an adipogenic sub-line of 3T3 fibroblasts (3T3-L1). 3T3-L1 fibroblasts are capable of undergoing differentiation to adipocytes under specific culture conditions. When compared to 3T3-L1 cells, the adipocyte differentiated cultures, which contain both pre-adipocytes and adipocytes, exhibited a significantly increased ability to support the growth of Ba/F3 cells expressing the chimeric receptor. Using probes specific for two recently described ligands for tie2, Ang-1 and Ang-2, we have shown that mRNA encoding Ang-1 is upregulated when 3T3-L1 fibroblasts are differentiated to adipocytes. These results suggest that the levels of Ang-1 protein and mRNA in 3T3-L1 cells can be regulated by cellular differentiation in adipose development.

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Citations

Nov 19, 2003·Biochemical and Biophysical Research Communications·Susan M DallabridaMaria A Rupnick
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ichiro SekiyaDarwin J Prockop
Jun 20, 2015·Mediators of Inflammation·Katsuhiko NaruseHiroshi Kobayashi
Apr 28, 2009·Advanced Drug Delivery Reviews·Zhihong Dong, Jacques E Nör
Nov 7, 2016·Tissue Engineering. Part a·Yu-Sheng HsuehFeng-Huei Lin
Feb 24, 2006·Journal of Neuroscience Research·Joanna KosackaKatharina Spanel-Borowski
Mar 30, 2001·The Journal of Biological Chemistry·M E BaldwinM G Achen

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