PMID: 7011070Jan 1, 1981Paper

The 48-hour exoerythrocytic stage of Plasmodium cynomolgi bastianellii

The American Journal of Tropical Medicine and Hygiene
W A KrotoskiD M Krotoski

Abstract

Detection and specific identification of the 48-hour exoerythrocytic stage of the primate malaria parasite, Plasmodium cynomolgi bastianellii, was accomplished by means of a highly specific indirect immunofluorescence technique (IFA) applied to hepatic tissue fixed in Carnoy's solution. The 48-hour forms appeared as round-to-slightly-oval bodies of average mean diameter 3.0 micrometers (9 parasites) and lying with the cytoplasm of individual hepatic parenchymal cells; each possessed one to three non-fluorescent nuclei or nuclear sections (mean 1.6) within the brightly fluorescent parasitic cytoplasm. In contrast, 72-hour parasites (6) had an average mean diameter of 4.0 micrometers and a mean of 2.2 nuclei. Restaining of IFA preparations with the Giemsa-colophonium method confirmed the plasmodial nature of fluorescent forms, despite some modification of staining characteristics produced by the prolonged exposure of sections to the aqueous phase of the IFA procedure. Exoerythrocytic forms could not be detected in biopsies obtained 24 hours following sporozoite inoculation.

Citations

Dec 25, 2013·Antimicrobial Agents and Chemotherapy·Anne-Marie ZeemanClemens H M Kocken
Jan 1, 1982·Transactions of the Royal Society of Tropical Medicine and Hygiene·M R Hollingdale, P Leland
Jan 1, 1992·Clinical Microbiology Reviews·F B Cogswell

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