PMID: 11332894May 3, 2001Paper

The 5-aminolaevulinic acid-based photodynamic effects on nuclei and nucleoli of HL-60 leukemic granulocytic precursors

Journal of Photochemistry and Photobiology. B, Biology
K SmetanaZ Hrkal

Abstract

To provide more information on the 5-aminolaevulinic acid (ALA)-based photodynamic effect (PDE) on nuclei and nucleoli of individual leukemic cells, these structures were studied in cultured HL-60 cells which originated from leukemic highly immature and less differentiated precursors of granulocytes. The nuclear morphology was visualized by panoptic May-Grünwald/Giemsa staining and cytochemical method for DNA, nucleoli were visualized by cytochemical methods for the demonstration of RNA and silver stainable proteins including those of interphase silver stained nucleolus organizer regions (AgNORs). In most cells ALA-based photodynamic treatment (PDT) produced marked alterations such as formation of apoptotic bodies, and large condensation of nuclear chromatin structure but without nuclear segmentation. Such changes are in harmony with the apoptotic process induced in these cells but without previous terminal differentiation. In nucleoli ALA-based PDT produced the reduction and disappearance of nucleolar silver stainable particles (SSPs) representing AgNORs which apparently reflected the alteration of the nucleolar biosynthetic activity and cell proliferation. The latter is also reflected by the disappearance of mitotic divisions...Continue Reading

References

Jan 1, 1984·Cell and Tissue Research·K Smetana, Z Likovský
Jan 1, 1997·Life Sciences·K SmetanaH Busch
Aug 26, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M BiggiogeraC Pellicciari
Mar 3, 1999·Journal of Photochemistry and Photobiology. B, Biology·D GrebenováZ Hrkal
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C Willingham

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Citations

Sep 6, 2005·Histochemistry and Cell Biology·Karel SmetanaZbynek Hrkal
Jul 20, 2005·Bulletin of Experimental Biology and Medicine·Yu S RomankoV V Popuchiev
Jan 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·K KuzelováZ Hrkal
Aug 9, 2002·Journal of Photochemistry and Photobiology. B, Biology·Karel SmetanaZbynek Hrkal
May 23, 2015·Journal of Photochemistry and Photobiology. B, Biology·Christian EweltMarkus Holling
Sep 2, 2005·Bulletin of Experimental Biology and Medicine·Yu S RomankoV V Popuchiev
Jun 18, 2020·Advanced Healthcare Materials·Wen PangXunbin Wei

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