PMID: 11924567Apr 2, 2002Paper

The 5' and 3' extremities of the satellite tobacco necrosis virus translational enhancer domain contribute differentially to stimulation of translation

RNA
R van LipzigF Meulewaeter

Abstract

The translational enhancer domain (TED) of satellite tobacco necrosis virus (STNV) RNA stimulates translation of uncapped RNAs autonomously. Here we set out to identify the 5' and 3' extremities of TED and features of these sequences with respect to translation. We found that both in wheat germ extract and in tobacco protoplasts, the 5' border is confined to 3 nt. Mutational analysis revealed that the autonomous function of TED is sensitive to 5' flanking sequences. At the 3' end of TED, 23 nt have a cumulative, quantitative effect on translation in wheat germ extract, whereas in tobacco protoplasts, the most 3' 14 nt of these 23 nt do not enhance translation. The 5' and 3' sequence requirements triggered the development of a new secondary structure model. In this model, TED folds into a phylogenetically conserved stem-loop structure in which the essential 5' nucleotides base-pair with the 3' nucleotides that stimulate translation both in vitro and in vivo. Importantly, the 14 3' nucleotides in TED that stimulate translation in the wheat germ extract only do not require the predicted base-pairing in order to function. The discrepancy between in vitro and in vivo sequence requirements thus correlates with potential base-pairing ...Continue Reading

References

Jun 30, 1995·Journal of Molecular Biology·A P GultyaevC W Pleij
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·F MeulewaeterM Cornelissen
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·P TranqueV P Mauro

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Citations

May 21, 2013·Annual Review of Microbiology·Anne E Simon, W Allen Miller
May 29, 2010·RNA·Beth L NicholsonK Andrew White
Nov 10, 2015·Molecular Plant-microbe Interactions : MPMI·Peter Palukaitis
Oct 9, 2015·Archives of Virology·Mart KrupovicMatthias G Fischer
Dec 20, 2005·Virus Research·Elizabeth L Pettit KnellerW Allen Miller
Jul 20, 2014·Bioorganic & Medicinal Chemistry Letters·Atsushi OgawaYasunori Doi
Dec 1, 2009·Viruses·Chung-Chi HuNa-Sheng Lin
Dec 15, 2017·Frontiers in Plant Science·Verónica TrunigerMiguel A Aranda
Apr 22, 2017·Frontiers in Plant Science·Manuel MirasMiguel A Aranda

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