PMID: 8610128Apr 2, 1996Paper

The 5' coding region of Paramecium surface antigen genes controls mutually exclusive transcription

Proceedings of the National Academy of Sciences of the United States of America
C L Leeck, J D Forney

Abstract

Paramecium tetraurelia stock 51 can express at least 11 different types of surface antigens, yet only a single type is expressed on the surface of an individual cell at any one time. The differential expression of stock 51 type A and B surface antigen genes (51A and 51B) is regulated at the level of transcription. Previously, we reported that nucleotide sequences upstream of position -26 (relative to the start of translation) in the 51A and 51B surface antigen genes are necessary for transcriptional activity but are not sufficient to direct differential transcriptional control. In this report we demonstrate that at least some of the critical elements necessary for differential transcription of the 51A and 51B genes lie within the 5' coding region. A hybrid gene that contains 51B upstream sequences (-475 to +1) attached to the ATG start codon of 51A is not cotranscribed with the 51B gene. In contrast, further substitution with 51B sequences (-1647 to +885) allows the chimeric gene to be coexpressed with 51B. A different hybrid gene containing a substitution of 51B sequence from -26 to +885 in the 51A gene is also coexpressed with 51B, revealing that the critical elements within the coding region of 51B do not require 51B upstrea...Continue Reading

References

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Citations

Aug 12, 1999·Acta histochemica·B A SalbillaS B Oppenheimer
Jul 21, 1999·Molecular and Biochemical Parasitology·K W DeitschT E Wellems
May 30, 2001·Trends in Genetics : TIG·P DessenJ Cohen
Jun 10, 2000·The Journal of Eukaryotic Microbiology·K Y Thai, J D Forney
Nov 12, 2002·The Journal of Eukaryotic Microbiology·Stephanie KervestinOlivier Jean-Jean
Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Hiroyuki TanabeSachiko Yoshioka
Jul 15, 2005·The Journal of Eukaryotic Microbiology·Martin C Simon, Helmut J Schmidt
Feb 16, 2007·The Journal of Eukaryotic Microbiology·Martin C Simon, Helmut J Schmidt
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Aug 18, 2004·Molecular and Cellular Biology·Olivier GarnierEric Meyer
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·K W DeitschT E Wellems

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