The 5' flanking regions of IRBP and arrestin have promoter activity in primary embryonic chicken retina cell cultures

Experimental Eye Research
Jeffrey H BoatrightJ M Nickerson

Abstract

Primary cultures of embryonic chicken cells from various tissues were transiently transfected with plasmid vectors containing reporter genes linked to a 1.8 kb fragment of the mouse interphotoreceptor retinoid-binding protein (IRBP) 5' flanking region, a 1.5 kb fragment of the mouse arrestin 5' flanking region, or a 3.4 kb sequence of the bovine arrestin 5' flanking region. Promoter activity was evident in retina-derived cells, but not in fibroblasts or cells from whole brain. Transfection response also varied with transfection method, plasmid DNA concentration, post-transfection incubation time, and cell density. The data suggest that the primary embryonic chicken retinal cell culture system is a useful tool in studying photoreceptor-specific gene regulation.

Citations

Feb 27, 2004·Investigative Ophthalmology & Visual Science·Elaine TanMuayyad R Al-Ubaidi
May 21, 1999·The Journal of Biological Chemistry·S S ManiB E Knox
Jan 8, 2000·The Journal of Biological Chemistry·A KimuraT Shinohara
Jan 3, 2001·Brain Research·J H BoatrightD E Borst

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