PMID: 6159579Jun 25, 1980Paper

The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae

Nucleic Acids Research
R Klemenz, E P Geiduschek

Abstract

The 5' terminus of Saccharomyces cereviasiae 35S pre rRNA was mapped on the rDNA using two methods: 1) Suitable restriction endonuclease fragments were hybridized to total high molecular weight RNA and extended with reverse transcriptase to the 5' end of the RNA template. 2) Other restriction fragments spanning the 5' terminus of 35S pre rRNA and radioactively labeled at their ends were hybridized to high molecular weight RNA and the non hybridized nucleic acids were digested with S1 nuclease. On the basis of these experiments, the 5' terminus of 35S pre rRNA was placed approximately 670 nucleotides upstream from the 17S rRNA coding region. The exact position was determined by reverse transcription as above, but in the presence of dideoxyribonucleoside triphosphates, which served as a way of sequencing the 5' terminal region. 35S pre rRNA synthesis is initiated at a site in EcoRI restriction fragment B which is 48 nucleotides upstream from the EcoRI cleavage site in the coding strand.

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Citations

Sep 7, 2004·Molecular Genetics and Genomics : MGG·R Prusty, R L Keil
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E O LongI B Dawid
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S A FuhrmanI M Verma
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I FinancsekM Muramatsu
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B D Kohorn, P M Rae
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B D Kohorn, P M Rae
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J L Teem, M Rosbash
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y NogiM Nomura
Jul 10, 1982·Nucleic Acids Research·R Miesfeld, N Arnheim
Oct 11, 1982·Nucleic Acids Research·O S Gabrielsen, T B Oyen
Nov 11, 1982·Nucleic Acids Research·J F BriatR Mache
Jul 11, 1984·Nucleic Acids Research·P O O'Connell, M Rosbash
Oct 21, 2014·Wiley Interdisciplinary Reviews. RNA·Antonio Fernández-PevidaJesús de la Cruz
Jan 5, 1988·Journal of Molecular Biology·L M Veinot-DrebotG C Johnston
Jul 16, 1981·Biochemical and Biophysical Research Communications·M SawadogoP Fromageot
Jun 1, 1989·Microbiological Reviews·J R Warner
Aug 1, 1989·Molecular and Cellular Biology·S E Stewart, G S Roeder
Jun 1, 1986·Molecular and Cellular Biology·E A Elion, J R Warner
Nov 1, 1989·Molecular and Cellular Biology·S P Johnson, J R Warner

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