The 7-transmembrane protein homologue UL78 of the human cytomegalovirus forms oligomers and traffics between the plasma membrane and different intracellular compartments

Archives of Virology
Svenja WagnerDetlef Michel

Abstract

The human cytomegalovirus (HCMV) UL78 ORF is considered to encode an orphan 7-transmembrane receptor. However, until now, the UL78 protein (pUL78) has not been characterized. Here, we have investigated the expression of pUL78 and found it mainly associated with the endoplasmic reticulum. However, we provide evidence that pUL78 is also localized on the cell surface from where it is quickly endocytosed. Colocalization with adaptin and EEA-1 implies that at least a small amount of pUL78 is transported to the trans Golgi network and early endosomes. Using a bimolecular fluorescence complementation assay and co-immunoprecipitation experiments, we were able to find homomeric and heteromeric structure formations of pUL78 and the US28 protein, respectively. However, the absence of pUL78 had no effect on the accumulation of inositol phosphate triggered by the US28 protein. In summary, our results suggest that the UL78 protein of HCMV traffics between the cell surface and cytoplasm, from where it might be recycled via early endosomes.

References

Jan 1, 1992·DNA and Cell Biology·W C ProbstS C Sealfon
Sep 1, 1997·Journal of Virology·S MichelsonM P Landini
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E PenfoldT J Schall
Oct 26, 2000·The Journal of Biological Chemistry·P CasarosaM J Smit
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S A Oliveira, T E Shenk
Oct 12, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·C VinkC A Bruggeman
Apr 15, 2003·The Journal of General Virology·Parvis AkterAndrew J Davison
Sep 25, 2003·The Biochemical Journal·Seyed Ali MousaviRune Kjeken
Jun 26, 2004·Annual Review of Neuroscience·Raul R GainetdinovMarc G Caron
Apr 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Beat NyfelerHans-Peter Hauri
Sep 21, 2005·The Journal of Biological Chemistry·Thomas PiechulekPeter Gierschik
Jun 3, 2006·Developmental Cell·Eric MaciaTomas Kirchhausen
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·David MaussangMartine J Smit
Aug 30, 2008·The Journal of Biological Chemistry·Claudia WalliserTom D Bunney
Mar 23, 2010·Trends in Pharmacological Sciences·Davide CalebiroMartin J Lohse
Oct 28, 2010·The Journal of Clinical Investigation·Gerold BongersSergio A Lira
Jun 21, 2011·Biochemical Pharmacology·Pia TschischeMaria Waldhoer

❮ Previous
Next ❯

Citations

Mar 26, 2015·Frontiers in Pharmacology·Sabrina M de MunnikHenry F Vischer
Mar 23, 2019·Medical Microbiology and Immunology·Theresa FrankThomas Stamminger
Dec 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maša MavriValentina Kubale
Mar 12, 2021·Pharmacological Reviews·Timo W M De GroofMartine J Smit

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
Anne Berit DyveK Sandvig
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
Chang XiaLan-qing Liu
© 2022 Meta ULC. All rights reserved