PMID: 2111804Jun 1, 1990Paper

The A-factor-binding protein of Streptomyces griseus negatively controls streptomycin production and sporulation

Journal of Bacteriology
K MiyakeT Beppu

Abstract

A-factor, 2-(6'-methylheptanoyl)-3R-hydroxymethyl-4-butanolide, is an autoregulator essential for streptomycin production and sporulation in Streptomyces griseus. S. griseus 2247 that requires no A-factor for streptomycin production or sporulation was found to have a defect in the A-factor-binding protein. This observation implied that the A-factor-binding protein in the absence of A-factor repressed the expression of both phenotypes in the wild-type strain. Screening among mutagenized S. griseus colonies for strains producing streptomycin and sporulating in the absence of A-factor yielded three mutants that were also deficient in the A-factor-binding protein. Reversal of the defect in the A-factor-binding protein of these mutants led to the simultaneous loss of streptomycin production and sporulation. These data suggested that the A-factor-binding protein played a role in repressing both streptomycin production and sporulation and that the binding of A-factor to the protein released its repression. Mutants deficient in the A-factor-binding protein began to produce streptomycin and sporulate at an earlier stage of growth than did the wild-type strain. These mutants produced approximately 10 times more streptomycin than did the ...Continue Reading

References

Aug 1, 1989·Journal of Bacteriology·K MiyakeT Beppu
Sep 1, 1982·The Journal of Antibiotics·O Hara, T Beppu
Dec 1, 1982·The Journal of Antibiotics·U GräfeL Radics

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Citations

Jan 1, 1990·Critical Reviews in Biotechnology·S Horinouchi, T Beppu
Jan 1, 1993·Antonie van Leeuwenhoek·S Horinouchi, T Beppu
Oct 13, 2016·Annual Review of Genetics·Juliane MacheleidtAxel A Brakhage
Jan 4, 2005·Biochemistry. Biokhimii︠a︡·S A Voloshin, A S Kaprelyants
Nov 1, 1995·Journal of Bacteriology·H OnakaS Horinouchi
Nov 1, 1995·Journal of Bacteriology·A LezhavaH Kinashi

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