PMID: 8971009Dec 1, 1996Paper

The a sequence is dispensable for isomerization of the herpes simplex virus type 1 genome

Journal of Virology
D W Martin, P C Weber

Abstract

The herpes simplex virus type 1 (HSV-1) genome consists of two components, L (long) and S (short), that invert relative to each other during productive infection to generate four equimolar isomeric forms of viral DNA. Recent studies have indicated that this genome isomerization is the result of DNA replication-mediated homologous recombination between the large inverted repeat sequences that exist in the genome, rather than site-specific recombination through the terminal repeat a sequences present at the L-S junctions. However, there has never been an unequivocal demonstration of the dispensability of the latter element for this process using a recombinant virus whose genome lacks a sequences at its L-S junctions. This is because the genetic manipulations required to generate such a viral mutant are not possible using simple marker transfer, since the cleavage and encapsidation signals of the a sequence represent essential cis-acting elements which cannot be deleted outright from the viral DNA. To circumvent this problem, a simple two-step strategy was devised by which essential cis-acting sites like the a sequence can be readily deleted from their natural loci in large viral DNA genomes. This method involved initial duplicati...Continue Reading

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Citations

Sep 15, 2005·Journal of Virology·Blair L Strang, Nigel D Stow
Jun 8, 2012·Journal of Virology·Charlotte MahietSébastien Barradeau
Aug 27, 2015·Virology Journal·Robyn N HallTimothy J Mahony
Nov 9, 2000·The Journal of Biological Chemistry·X D Yao, P Elias
Mar 26, 2002·The Journal of Biological Chemistry·Ke-Jung HuangI Robert Lehman
Sep 10, 1999·Reviews in Medical Virology·K Umene

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