The A2B adenosine receptor colocalizes with adenosine deaminase in resting parietal cells from gastric mucosa

Biochemistry. Biokhimii︠a︡
R M ArinB Ochoa

Abstract

The A2B adenosine receptor (A2BR) mediates biological responses to extracellular adenosine in a wide variety of cell types. Adenosine deaminase (ADA) can degrade adenosine and bind extracellularly to adenosine receptors. Adenosine modulates chloride secretion in gastric glands and gastric mucosa parietal cells. A close functional link between surface A2BR and ADA has been found on cells of the immune system, but whether this occurs in the gastrointestinal tract is unknown. The goal of this study was to determine whether A2BR and ADA are coexpressed at the plasma membrane of the acid-secreting gastric mucosa parietal cells. We used isolated gastric parietal cells after purification by centrifugal elutriation. The membrane fraction was obtained by sucrose gradient centrifugation. A2BR mRNA expression was analyzed by RT-PCR. The surface expression of A2BR and ADA proteins was evaluated by Western blotting, flow cytometry and confocal microscopy. Our findings demonstrate that A2BR and ADA are expressed in cell membranes isolated from gastric parietal cells. They show a high degree of colocalization that is particularly evident in the surface of contact between parietal cells. The confocal microscopy data together with flow cytometr...Continue Reading

References

Feb 1, 1976·Acta Physiologica Scandinavica·T Berglindh, K J Obrink
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M AranR Franco
Mar 1, 1990·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·C E Gil-RodrigoL F Ainz
Jun 1, 1989·Pflügers Archiv : European journal of physiology·H SakaiN Takeguchi
Jan 1, 1994·Annual Review of Physiology·C S Chew
May 1, 1993·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·L F AinzC E Gil-Rodrigo
Jul 1, 1997·Progress in Neurobiology·R FrancoC Lluis
Apr 29, 1998·Immunological Reviews·R FrancoJ Blanco
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H A OlanrewajuS J Mustafa
Mar 30, 2001·The Journal of Biological Chemistry·C HerreraR Franco
Jan 5, 2002·The Biochemical Journal·Silvia GinésRafael Franco
Jan 27, 2004·The Journal of Pharmacology and Experimental Therapeutics·Linda Yip, Yin Nam Kwok
Jul 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lixin WangShanthi V Sitaraman
May 3, 2005·Experimental Eye Research·Kah Y Tan-AllenJoseph A Bonanno
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·R PachecoR Franco
Nov 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tianqing KongSean P Colgan
Jan 27, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lynne A LapierreJames R Goldenring
Aug 31, 2007·Expert Opinion on Biological Therapy·Annemie Van Linden, Holger K Eltzschig
Feb 12, 2009·The Journal of Pharmacology and Experimental Therapeutics·Gary K YangYin Nam Kwok
Feb 4, 2010·World Journal of Gastroenterology : WJG·Sherif M Karam
Jun 16, 2010·Biochimica Et Biophysica Acta·Carol M AherneHolger K Eltzschig
Nov 30, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eduard GraciaVicent Casadó
Jan 5, 2013·Biochemistry·Dominik ThimmChrista E Müller
Jan 9, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sean P ColganStefan F Ehrentraut
Feb 12, 2014·Biochemistry. Biokhimii︠a︡·R M ArinB Ochoa

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Citations

Oct 20, 2015·Biochemical and Biophysical Research Communications·Ping WangShuixiang He
Sep 9, 2016·Frontiers in Chemistry·Ying Sun, Pingbo Huang
Apr 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rosa María ArinBegoña Ochoa
Nov 2, 2019·Physiological Reviews·Amy C EngevikJames R Goldenring
Oct 12, 2017·Frontiers in Physiology·Rosa M ArinBegoña Ochoa
Mar 3, 2018·Frontiers in Pharmacology·Estefanía MorenoVicent Casadó
Oct 16, 2015·American Journal of Physiology. Cell Physiology·Rosa María ArinBegona Ochoa
Sep 15, 2019·International Journal of Biological Macromolecules·S BagheriT Haertlé

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