The A2B adenosine receptor in MDA-MB-231 breast cancer cells diminishes ERK1/2 phosphorylation by activation of MAPK-phosphatase-1

PloS One
Marthe KoussémouKarl-Norbert Klotz

Abstract

It was previously shown that the estrogen-receptor negative breast cancer cell line MBA-MD-231 expresses high levels of A2B adenosine receptors as the sole adenosine receptor subtype. These receptors couple to both, stimulation of adenylyl cyclase and a Ca2+ signal. In order to establish a potential role of A2B adenosine receptors in tumor growth and development MAPK signaling was investigated in these breast cancer cells. Although it is known that A2B adenosine receptors may stimulate MAPK it was found that in MBA-MD-231 cells ERK1/2 phosphorylation is reduced upon agonist-stimulation of A2B adenosine receptors. This reduction is also triggered by forskolin, but abolished by the PKA inhibitor H89, suggesting an important role for the cAMP-PKA pathway. Likewise, a role for intracellular Ca2+ was established as the Ca2+ chelator 1,2-bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester (BAPTA-AM) abolished the reduction of ERK1/2 phosphorylation triggered by A2B stimulation. It was shown that various pathways downstream from A2B adenosine receptors resulted in a stimulation of MAPK phosphatase-1 (MKP-1) which dephosphorylates phospho ERK1/2, and thus plays a critical role in the regulation of the phosp...Continue Reading

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Citations

Feb 11, 2020·International Reviews of Immunology·Marharyta Zyma, Rafał Pawliczak
Oct 20, 2019·International Journal of Molecular Sciences·Zhan-Guo Gao, Kenneth A Jacobson
Apr 16, 2019·Frontiers in Cellular Neuroscience·Kenneth A JacobsonZhan-Guo Gao
Jul 25, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Marthe Koussémou, Karl-Norbert Klotz
May 14, 2021·Purinergic Signalling·M Reyna-JeldesF G Vázquez-Cuevas

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

GraphPad
ImageJ
GraphPad Prism

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