The A3 adenosine receptor agonist 2-Cl-IB-MECA facilitates epileptiform discharges in the CA3 area of immature rat hippocampal slices

Epilepsy Research
Mark Anthony Laudadio, Caterina Psarropoulou

Abstract

The effects of the A(3) adenosine receptor agonist 2-Cl-IB-MECA were tested on epileptiform field potentials recorded in the CA3 area of postnatal days 10-20 immature hippocampal slices, during perfusion with the GABA(A) receptor antagonist bicuculline (10 microM). Evoked potentials: 2-Cl-IB-MECA (1-50 microM, n = 17) had consistently excitatory effects, blocked by the A(3) receptor antagonist MRS 1220 (1 microM, n = 7), but not occluded in the presence of the A(1) antagonist DPCPX (1 microM, n = 12) or the A(2A) antagonist ZM-241385 (0.1 microM, n = 12). 2-Cl-IB-MECA reversed the inhibitory effects (n = 5) of the adenosine uptake blocker nitrobenzylthioinosine (NBTI, 50 microM), but did not increase its excitatory effects (n = 19). Spontaneous discharges: 2-Cl-IB-MECA (1 microM) induced them or increased their frequency in 14/30 slices, an effect reversed by MRS 1220 (n = 3), and observed also following pre-perfusion with DPCPX (n = 11), ZM-241385 (n = 11) or both (n = 10). In the presence of the A(1) antagonist DPCPX, NBTI increased the frequency of spontaneous discharges, an effect partially reversed by MRS 1220 (n = 8), thus suggesting that a rise in endogenous adenosine during disinhibition may activate A(3) receptors. In ...Continue Reading

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Aug 5, 2003·Experimental Brain Research·Caterina PsarropoulouMark Anthony Laudadio

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Citations

Nov 22, 2007·Pharmacology & Therapeutics·Stefania GessiPier Andrea Borea
Mar 6, 2015·European Journal of Nuclear Medicine and Molecular Imaging·D HaeuslerMarkus Mitterhauser
Jul 17, 2019·Current Pharmaceutical Design·Hira ChoudhuryBapi Gorain
Mar 28, 2020·Cells·Wiwin Is EffendiYoshihiro Nishimura
Jun 23, 2020·Journal of Caffeine and Adenosine Research·Fabio C TescarolloDetlev Boison
Dec 5, 2016·Pharmacological Reports : PR·Marek CieślakMichał Komoszyński

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