The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-XL: studies in human astroglioma cells

Biochemical and Biophysical Research Communications
Maria P AbbracchioW Malorni

Abstract

The pathophysiological role of the adenosine A3 receptor in the central nervous system is largely unknown. We have investigated the effects of the selective A3 receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine, Cl-IB-MECA, in cells of the astroglial lineage (human astrocytoma ADF cells). A marked reorganization of the cytoskeleton, with appearance of stress fibers and numerous cell protrusions, was found following exposure of cells to low (nM) concentrations of Cl-IB-MECA. These "trophic" effects were accompanied by induction of the expression of Rho, a small GTP-binding protein, which was virtually absent in control cells, and by changes of the intracellular distribution of the antiapoptotic protein Bcl-XL, that, in agonist-exposed cells, became specifically associated to cell protrusions. This is the first demonstration that the intracellular organization of Bcl-XL can be modulated by the activation of a G-protein-coupled membrane receptor, such as the A3 adenosine receptor. Moreover, modulation of the astrocytic cytoskeleton by adenosine may have intriguing implications in both nervous system development and in the response of the brain to trauma and ischemia.

Citations

Apr 12, 2008·Purinergic Signalling·Stefania MerighiPier Andrea Borea
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Jul 4, 1998·Trends in Pharmacological Sciences·K A Jacobson
Apr 3, 1999·British Journal of Pharmacology·R BrambillaM P Abbracchio
Feb 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhoumou ChenDaniela Salvemini
Feb 11, 2000·Annals of the New York Academy of Sciences·M P Abbracchio, F Cattabeni
Feb 11, 2000·Annals of the New York Academy of Sciences·D K von LubitzR C Lin
Mar 23, 2012·Expert Opinion on Therapeutic Patents·Stephanie Federico, Giampiero Spalluto
Nov 22, 2014·Brain : a Journal of Neurology·Joshua W LittleDaniela Salvemini
Feb 7, 2013·Neurochemistry International·Eleonora AronicaDetlev Boison
Oct 4, 2006·Journal of Neuroscience Research·Guann-Juh ChenYun Wang
Nov 13, 2014·Pharmacological Reviews·Pier Andrea BoreaStefania Gessi
Jun 18, 2018·Pharmacological Reports : PR·Amira M SolimanAhmed A Moustafa
Dec 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Susan MozzicatoBruce T Liang
Mar 19, 2020·Pain·Kenneth A JacobsonDaniela Salvemini
Jun 21, 2016·Journal of Neuroscience Research·Heng YanXin Zhao
Jul 21, 2001·Annals of the New York Academy of Sciences·M P AbbracchioC Fiorentini
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hays W J YoungMichael R Blackburn
Jan 26, 2016·British Journal of Pharmacology·K JanesD Salvemini
Jul 21, 2001·Annals of the New York Academy of Sciences·D K Von LubitzR C Lin
Apr 23, 2002·Glia·Patrizia Di IorioMichel P Rathbone
Oct 28, 2020·International Journal of Molecular Sciences·Aida Menéndez MéndezTobias Engel
Sep 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stefania GessiPier Andrea Borea
Dec 1, 2004·Journal of Neurochemistry·Maria L TrincavelliClaudia Martini
Mar 26, 2002·Biochemical Pharmacology·Seong Gon KimKenneth A Jacobson
Feb 13, 1999·European Journal of Pharmacology·D K Von Lubitz
Jun 4, 1999·European Journal of Pharmacology·D K von Lubitz
Jun 14, 2000·Progress in Neurobiology·M P RathboneF Caciagli
Jun 3, 2021·Journal of Neuroscience Research·Heather Leduc-PessahTuan Trang
Jul 2, 2003·Cellular Signalling·Gunnar Schulte, Bertil B Fredholm
Nov 22, 2007·Pharmacology & Therapeutics·Stefania GessiPier Andrea Borea

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