The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome.

Proceedings of the National Academy of Sciences of the United States of America
Aliaa H AbdelhakimTania A Baker

Abstract

A hyperstable complex of the tetrameric MuA transposase with recombined DNA must be remodeled to allow subsequent DNA replication. ClpX, a AAA+ enzyme, fulfills this function by unfolding one transpososome subunit. Which MuA subunit is extracted, and how complex destabilization relates to establishment of the correct directionality (left to right) of Mu replication, is not known. Here, using altered-specificity MuA proteins/DNA sites, we demonstrate that transpososome destabilization requires preferential ClpX unfolding of either the catalytic-left or catalytic-right subunits, which make extensive intersubunit contacts in the tetramer. In contrast, ClpX recognizes the other two subunits in the tetramer much less efficiently, and their extraction does not substantially destabilize the complex. Thus, ClpX targets the most stable structural components of the complex. Left-end biased Mu replication is not, however, determined by ClpX's intrinsic subunit preference. The specific targeting of a stabilizing "keystone subunit" within a complex for unfolding is an attractive general mechanism for remodeling by AAA+ enzymes.

References

Mar 7, 1977·Molecular & General Genetics : MGG·C Wijffelman, B Lotterman
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M MizuuchiK Mizuuchi
Jul 30, 1985·Virology·M A Groenen, P van de Putte
Oct 1, 1995·Genes & Development·I LevchenkoT A Baker
Aug 18, 1995·The Journal of Biological Chemistry·H Nakai, R Kruklitis
Jul 22, 1998·Nucleic Acids Research·S Y NamgoongR M Harshey
Jul 14, 1999·Nature·S G WolfA Minsky
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·H NakaiJ M Jones
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Saskia B NeherTania A Baker
Apr 23, 2004·Current Opinion in Structural Biology·Mindy Steiniger-WhiteWilliam S Reznikoff
Jul 28, 2005·Protein Science : a Publication of the Protein Society·Briana M Burton, Tania A Baker
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sean D MooreRobert T Sauer

❮ Previous
Next ❯

Citations

Dec 12, 2012·Quarterly Reviews of Biophysics·Fred DydaAlison Burgess Hickman
Nov 9, 2012·Nature·Sherwin P MontañoPhoebe A Rice
Oct 7, 2011·Nucleic Acids Research·Slawomir KubikIgor Konieczny
Dec 12, 2012·Mobile DNA·Rasika M Harshey
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wonyoung ChoiRasika M Harshey
Mar 29, 2011·Current Opinion in Structural Biology·Sherwin P Montaño, Phoebe A Rice
Sep 27, 2014·Molecular Microbiology·Wonyoung ChoiRasika M Harshey
Mar 24, 2015·Journal of Molecular Biology·Lorraine LingTania A Baker
May 6, 2015·Nature Structural & Molecular Biology·Bei YangJames H Hurley
Aug 21, 2013·The Journal of Biological Chemistry·Matthew A HumbardMichael R Maurizi
Oct 4, 2014·The Journal of Biological Chemistry·Yoshiharu SatoTomoko Yamamoto
Jun 25, 2015·Microbiology Spectrum·Rasika M Harshey
Jun 25, 2015·Microbiology Spectrum·Alison B Hickman, Fred Dyda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Wonyoung Choi, R M Harshey
Proceedings of the National Academy of Sciences of the United States of America
Hiroshi NakaiJ M Jones
Annual Review of Biochemistry
Nigel D F GrindleyPhoebe A Rice
© 2021 Meta ULC. All rights reserved