The AAE14 gene encodes the Arabidopsis o-succinylbenzoyl-CoA ligase that is essential for phylloquinone synthesis and photosystem-I function

The Plant Journal : for Cell and Molecular Biology
Hyun Uk KimJohn Browse

Abstract

Phylloquinone is the one-electron carrier at the A(1) site of photosystem I, and is essential for photosynthesis. Arabidopsis mutants deficient in early steps of phylloquinone synthesis do not become autotrophic and are seedling lethals, even when grown on sucrose-supplemented media. Here, we identify acyl-activating enzyme 14 (AAE14, At1g30520) as the o-succinylbenzoyl-coenzyme A (OSB-CoA) ligase acting in phylloquinone synthesis. Three aae14 mutant alleles, identified by reverse genetics, were found to be seedling lethal, to contain no detectable phylloquinone (< 0.1 pmol mg(-1) fresh weight) compared with 10 pmol mg(-1) fresh weight in wild-type leaves, and to accumulate OSB. AAE14 was able to restore menaquinone biosynthesis when expressed in an Escherichia coli mutant disrupted in the menE gene that encodes the bacterial OSB-CoA ligase. Weak expression of an AAE14 transgene in mutant plants (controlled by the uninduced XVE promoter) resulted in chlorotic, slow-growing plants that accumulated an average of 4.7 pmol mg(-1) fresh weight of phylloquinone. Inducing the XVE promoter in these plants, or expressing an AAE14 transgene under the control of the CaMV 35S promoter, led to full complementation of the mutant phenotype. a...Continue Reading

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Citations

Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Joshua R WidhalmGilles J C Basset
Mar 1, 2012·Journal of Experimental Botany·Lucia Eugeni PillerCéline Besagni
May 3, 2008·Plant Physiology·Christophe GarcionJean-Pierre Métraux
Mar 1, 2012·The Plant Cell·Teresa B FitzpatrickAlisdair R Fernie
Apr 25, 2008·BMC Evolutionary Biology·Jeferson GrossDebashish Bhattacharya
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Jay Shockey, John Browse
Jul 16, 2010·The Plant Journal : for Cell and Molecular Biology·Fabienne FurtGilles J C Basset
Jan 15, 2015·Biochimica Et Biophysica Acta·Huixia YangCongming Lu
Aug 9, 2016·Current Opinion in Plant Biology·Sigrun Reumann, Bonnie Bartel
Feb 20, 2018·Bioscience, Biotechnology, and Biochemistry·Makoto Kawamukai
Nov 6, 2018·Horticulture Research·Rachel M McCoyJoshua R Widhalm
Apr 24, 2018·Frontiers in Molecular Biosciences·Anutthaman ParthasarathyAndré O Hudson

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