The ABC transporter BcatrB affects the sensitivity of Botrytis cinerea to the phytoalexin resveratrol and the fungicide fenpiclonil

Molecular Plant-microbe Interactions : MPMI
H SchoonbeekM A De Waard

Abstract

During pathogenesis, fungal pathogens are exposed to a variety of fungitoxic compounds. This may be particularly relevant to Botrytis cinerea, a plant pathogen that has a broad host range and, consequently, is subjected to exposure to many plant defense compounds. In practice, the pathogen is controlled with fungicides belonging to different chemical groups. ATP-binding cassette (ABC) transporters might provide protection against plant defense compounds and fungicides by ATP-driven efflux mechanisms. To test this hypothesis, we cloned BcatrB, an ABC transporter-encoding gene from B. cinerea. This gene encodes a 1,439 amino acid protein with nucleotide binding fold (NBF) and transmembrane (TM) domains in a [NBF-TM6]2 topology. The amino acid sequence has 31 to 67% identity with ABC transporters from various fungi. The expression of BcatrB is up regulated by treatment of B. cinerea germlings with the grapevine phytoalexin resveratrol and the fungicide fenpiclonil. BcatrB replacement mutants are not affected in saprophytic growth on different media but are more sensitive to resveratrol and fenpiclonil than the parental isolate. Furthermore, virulence of deltaBcatrB mutants on grapevine leaves was slightly reduced. These results in...Continue Reading

References

May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W Chen, K Struhl
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·E Balzi, A Goffeau
Jul 1, 1994·Molecular and Cellular Biology·D J KatzmannW S Moye-Rowley
Feb 1, 1994·Current Genetics·U W HilberW Köller
Dec 27, 1995·FEBS Letters·A E SeniorI L Urbatsch
Jan 1, 1997·Molecular Plant-microbe Interactions : MPMI·J A van KanC J van der Vlugt-Bergmans
Feb 1, 1997·Nature Genetics·A Decottignies, A Goffeau
Apr 28, 1997·Molecular & General Genetics : MGG·G Del SorboM A De Waard
Jan 27, 1998·The Journal of Biological Chemistry·T C Hallstrom, W S Moye-Rowley
Nov 18, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M KolaczkowskiA Goffeau
Jun 11, 1999·Fungal Genetics and Biology : FG & B·A E Osbourn
Aug 24, 2000·Fungal Genetics and Biology : FG & B·G Del SorboM A De Waard

❮ Previous
Next ❯

Citations

Mar 1, 2012·The Journal of Microbiology·Hyosun Cho, Hyojeung Kang
Jul 20, 2002·Phytochemistry·Alfred M Mayer, Richard C Staples
Nov 19, 2003·International Journal of Food Microbiology·Belén López-GarcíaJose F Marcos
Jun 14, 2002·The Plant Journal : for Cell and Molecular Biology·Sybille van den BrûleCheryl C Smart
Jul 1, 2001·Molecular Plant Pathology·A Idnurm, B J Howlett
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Joseph D ReddyDean W Gabriel
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Julia SchumacherBettina Tudzynski
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·C S GronoverB Tudzynski
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Henk-jan SchoonbeekMaarten A De Waard
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Ioannis StergiopoulosMaarten A De Waard
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Antje BurseMatthias S Ullrich
Nov 27, 2009·Canadian Journal of Microbiology·Zhifeng CuiTingheng Zhu
May 6, 2003·Annual Review of Phytopathology·Brion DuffyJos M Raaijmakers
Nov 27, 2002·Bioscience, Biotechnology, and Biochemistry·Noriyuki OchiaiIsamu Yamaguchi
Aug 21, 2012·PloS One·Angela M OrshinskyMichael J Boehm
Jun 27, 2009·PLoS Pathogens·Jeffrey J Coleman, Eleftherios Mylonakis
Apr 3, 2010·PLoS Pathogens·Heather C Rowe, Daniel J Kliebenstein
Nov 8, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Philippe JeandetJérôme Crouzet
Nov 23, 2006·Phytochemistry·Elisa BarileVirginia Lanzotti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.