The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120

Life
Dmitry ShvarevIris Maldener

Abstract

Anabaena sp. PCC 7120 is a filamentous cyanobacterium able to fix atmospheric nitrogen in semi-regularly spaced heterocysts. For correct heterocyst function, a special cell envelope consisting of a glycolipid layer and a polysaccharide layer is essential. We investigated the role of the genes hgdB and hgdC, encoding domains of a putative ABC transporter, in heterocyst maturation. We investigated the subcellular localization of the fusion protein HgdC-GFP and followed the differential expression of the hgdB and hgdC genes during heterocyst maturation. Using a single recombination approach, we created a mutant in hgdB gene and studied its phenotype by microscopy and analytical chromatography. Although heterocysts are formed in the mutant, the structure of the glycolipid layer is aberrant and also contains an atypical ratio of the two major glycolipids. As shown by a pull-down assay, HgdB interacts with the outer membrane protein TolC, which indicates a function as a type 1 secretion system. We show that the hgdB-hgdC genes are essential for the creation of micro-oxic conditions by influencing the correct composition of the glycolipid layer for heterocyst function. Our observations confirm the significance of the hgdB-hgdC gene cl...Continue Reading

References

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Citations

Feb 20, 2020·The Journal of General and Applied Microbiology·Tsukasa Saito, Koichiro Awai
Jun 10, 2020·Journal of Basic Microbiology· Harish, Kunal Seth
May 15, 2019·Life·Antonia Herrero, Enrique Flores

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Methods Mentioned

BETA
pull-down
PCR
electrophoresis
light microscopy
fluorescence microscopy

Software Mentioned

GelQuantNET
Multalign
GraphPad Prism
Leica ASF
BLAST

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