The ABD on the nascent polypeptide and PH domain are required for the precise Anillin localization in Drosophila syncytial blastoderm.

Scientific Reports
Tomoki HirashimaHideki Yoshida

Abstract

Targeting proteins to regions where they are required is essential for proper development of organisms. For achievement of this, subcellular mRNA localization is one of the critical mechanisms. Subcellular mRNA localization is an evolutionarily conserved phenomenon from E. coli to human and contributes to limiting the regions at which its products function and efficiently supplies substrates for protein translation. During early Drosophila embryogenesis, while 71% of the 3370 mRNAs analyzed have shown prominent subcellular localization, the underlying molecular mechanisms have not been elucidated. Here, we reveal that anillin mRNA, one of the localized mRNAs in early Drosophila embryo, localizes to the tip of the pseudo-cleavage furrow in the Drosophila syncytial blastoderm using in situ hybridization combined with immunohistochemistry. Localization analyses with transgenic fly lines carrying a series of deletion mRNAs indicate that this localization is dependent on its own nascent polypeptides including the actin binding domain (ABD). In addition to the mRNA localization, it is revealed that the pleckstrin homology (PH) domain of Anillin protein is also required for its proper localization. Thus, we indicate that the precise l...Continue Reading

References

Oct 1, 1983·Developmental Biology·W R JefferyR D Brodeur
Oct 1, 1995·The Journal of Cell Biology·C M Field, B M Alberts
Feb 1, 1995·Current Opinion in Cell Biology·W Sullivan, W E Theurkauf
Jan 1, 1993·Annual Review of Cell Biology·E D Schejter, E Wieschaus
Dec 1, 1995·Molecular Biology of the Cell·H FaresJ R Pringle
Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Aveek Mazumdar, Manjari Mazumdar
Jun 27, 2008·Molecular Biology of the Cell·Rosalind V Silverman-GavrilaAndrew Wilde
Feb 14, 2009·International Review of Cell and Molecular Biology·Afsar U Ahmed, Paul R Fisher
Jul 28, 2011·The EMBO Journal·Cornelia Kilchert, Anne Spang
Dec 27, 2011·Current Biology : CB·Jinghe LiuAndrew Wilde
Jun 4, 2013·Molecular Cell·Erik GutierrezThomas E Dever
May 6, 2016·Molecular Biology of the Cell·Aparna Sherlekar, Richa Rikhy

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

CFX Manager

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.