The ability of human Schwann cell grafts to promote regeneration in the transected nude rat spinal cord

Experimental Neurology
J D GuestR P Bunge

Abstract

Advances in the purification and expansion of Schwann cells (SCs) from adult human peripheral nerve, together with biomaterials development, have made the construction of unique grafts with defined properties possible. We have utilized PAN/PVC guidance channels to form solid human SC grafts which can be transplanted either with or without the channel. We studied the ability of grafts placed with and without channels to support regeneration and to influence functional recovery; characteristics of the graft and host/graft interface were also compared. The T9-T10 spinal cord of nude rats was resected and a graft was placed across the gap; methylprednisolone was delivered acutely to decrease secondary injury. Channels minimized the immigration of connective tissue into grafts but contributed to some necrotic tissue loss, especially in the distal spinal cord. Grafts without channels contained more myelinated axons (x = 2129 +/- 785) vs (x = 1442 +/- 514) and were larger in cross-sectional area ( x = 1.53 +/- 0.24 mm2) vs (x = 0.95 +/- 0.86 mm2). The interfaces formed between the host spinal cord and the grafts placed without channels were highly interdigitated and resembled CNS-PNS transition zones; chondroitin sulfate proteoglycans...Continue Reading

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Citations

Mar 2, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Marie-Pascale CôtéJohn D Houle
Oct 18, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Michael G Fehlings, Reaz Vawda
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