PMID: 7537262Mar 1, 1995Paper

The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria

The Journal of Applied Bacteriology
D J MasonD Lloyd

Abstract

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

References

Nov 1, 1992·Applied Microbiology and Biotechnology·J P DiaperC Edwards
Apr 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·C A RahnD J Doolittle
Mar 30, 1990·Biochimica Et Biophysica Acta·Z LakosS Damjanovich
Dec 1, 1989·Biophysical Journal·D L FarkasL M Loew
May 15, 1984·Archives of Biochemistry and Biophysics·A PeñaM Borbolla
Apr 11, 1984·Biochimica Et Biophysica Acta·T BräunerR J Strasser
Jan 1, 1993·Biology of the Cell·M TroussellierA Vaquer
Nov 1, 1938·The Journal of Hygiene·A A MilesJ O Irwin

❮ Previous
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Citations

Dec 22, 2004·Archives of Microbiology·Christian LaflammeCaroline Duchaine
Jun 27, 2009·Journal of Industrial Microbiology & Biotechnology·Jaume Comas-Riu, Núria Rius
Apr 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·J Plásek, K Sigler
Sep 15, 2000·Journal of Immunological Methods·D A VealP V Attfield
May 3, 2000·International Journal of Food Microbiology·P Breeuwer, T Abee
Sep 9, 2000·FEMS Microbiology Reviews·J Vives-RegoG Nebe-von Caron
Oct 1, 1995·The Journal of Applied Bacteriology·J PorterR W Pickup
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Pier Carlo BragaMonica Dal Sasso
Aug 31, 2010·Applied and Environmental Microbiology·Eduardo SánchezNorma Heredia
Sep 30, 2000·Applied and Environmental Microbiology·S E CowanJ D Keasling
Jan 7, 2003·Applied and Environmental Microbiology·W HaidingerW Lubitz
Jul 8, 2005·Applied and Environmental Microbiology·Pilar Delgado-ViscogliosiJean Marie Delattre
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Jan 14, 2012·BMC Microbiology·Raja VukantiLaura G Leff
Jun 3, 2009·Journal of Applied Microbiology·U P Cronin, M G Wilkinson
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
Oct 26, 2016·Journal of Microbiological Methods·Janine McGowanErnesto Guzman-Novoa
Sep 5, 2008·Current Protocols in Cytometry·I D Johnson
Sep 5, 2008·Current Protocols in Cytometry·Hazel M DaveyArseny S Kaprelyants
Apr 6, 2001·Biotechnology and Bioengineering·R DaugelaviciusD H Bamford
May 27, 2008·Cytometry. Part B, Clinical Cytometry·Tania FalcioniWally Baffone
Dec 31, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·R W SabnisT S Dalvi
Sep 5, 2008·Current Protocols in Cytometry·Jonathan Porter
Jan 5, 2002·Journal of Food Protection·K Takeuchi, J F Frank
Nov 13, 2018·MSystems·Jingwei CaiAndrew D Patterson
Apr 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·James P StratfordMunehiro Asally
Feb 17, 1998·Applied and Environmental Microbiology·M VirtaE M Lilius

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