The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions

Frontiers in Microbiology
Annel M Hernández-AlcántaraPaloma López

Abstract

Riboflavin, vitamin B2, is essential for humans and has to be obtained from the diet. Some lactic acid bacteria (LAB) produce this vitamin, and they can be used for in-situ fortification of foods. This could be an alternative to supplementation with chemically synthesized vitamin, to palliate riboflavin deficiencies in specific groups of people. Moreover, if the producing LAB could survive in the gastrointestinal stress (GIT) they could be added as probiotics in this environment. In the present study we tested two riboflavin-overproducing Lactiplantibacillus plantarum strains (M5MA1-B2 and M9MG6-B2), spontaneous mutants of LAB isolated from chicha, a traditional Andean beverage. These two LAB, and also their isogenic strains M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12], expressing the mCherry protein from the pRCR12 plasmid, were evaluated in vitro under simulated GIT conditions. Among other, specifically developed protein fluorescence assays were used. The four LAB showed similar levels of adhesion (>6.0%) to Caco-2 cells, higher than that of the probiotic Lacticaseibacillus rhamnosus GG strain (4.51%). Thus, LAB biofilm formation was assessed in the labeled cells by intracellular mCherry fluorescence and in the unlabeled parental st...Continue Reading

References

Mar 1, 1997·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·M J Hill
Apr 9, 2001·Journal of Bacteriology·C CucarellaJ R Penadés
Sep 26, 2001·Applied and Environmental Microbiology·A Toledo-AranaI Lasa
Mar 15, 2003·European Journal of Nutrition·Albert FlynnValérie Rolland
Jun 7, 2003·The American Journal of Clinical Nutrition·Hilary J Powers
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Paul D Cotter, Colin Hill
Jan 20, 2004·Proteomics·Maria De Angelis, Marco Gobbetti
Oct 7, 2004·Applied and Environmental Microbiology·Catherine BurgessDouwe van Sinderen
Aug 17, 2005·FEMS Microbiology Reviews·Máire BegleyColin Hill
Jul 20, 2006·Microbial Cell Factories·Catherine M BurgessDouwe van Sinderen
Jan 30, 2007·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Miguel Gueimonde, Seppo Salminen
Aug 19, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Srdjan StepanovićFilip Ruzicka
Dec 25, 2007·Applied and Environmental Microbiology·Claudia SánchezHelena Santos
Jul 29, 2008·Folia Microbiologica·E VlkováJ Killer
Sep 5, 2008·Current Protocols in Microbiology·Judith H MerrittGeorge A O'Toole
Mar 9, 2010·Journal of Bacteriology·Jeff R BroadbentJames L Steele
Jun 18, 2011·Journal of Agricultural and Food Chemistry·Vittorio CapozziGiuseppe Spano
Dec 27, 2011·The Indian Journal of Medical Research·Raj Kumar DuarySunita Grover
Mar 22, 2014·The British Journal of Nutrition·Marziyeh Ashoori, Ahmad Saedisomeolia
Jun 7, 2014·Applied Microbiology and Biotechnology·Mattia P ArenaGiuseppe Spano
Jan 15, 2015·Applied Microbiology and Biotechnology·Pasquale RussoGiuseppe Spano
Apr 1, 2015·FEMS Microbiology Letters·Najla HaddajiAmina Bakhrouf
May 13, 2015·International Journal of Food Microbiology·Mónica D Fernández RamírezMasja N Nierop Groot
Nov 5, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aimee ParkerCarmen Pin
Feb 12, 2019·Journal of Clinical Gastroenterology·Lucio Capurso
Jul 4, 2019·Applied Microbiology and Biotechnology·Andrea Monteagudo-MeraAfroditi Chatzifragkou
Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
Feb 15, 2020·Microbial Cell Factories·Shuang LiuTao Chen
Mar 1, 2020·Applied Microbiology and Biotechnology·Jean Guy LeBlancAlejandra de Moreno de LeBlanc
Oct 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fernanda B HaffnerAndreea Pasc

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
fluorescence microscopy

Software Mentioned

Leica Application Suite X
MINITAB
AxioVision
R

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.