The ability of the BANA Test to detect different levels of P. gingivalis, T. denticola and T. forsythia

Brazilian Oral Research
José Alexandre de AndradeSheila Cavalca Cortelli

Abstract

The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chro...Continue Reading

References

May 1, 1988·Journal of Clinical Periodontology·J L DzinkA D Haffajee
Jan 1, 1995·Journal of Periodontology·S G GrossiE Hausmann
Apr 1, 1994·Analytical Biochemistry·R P AldertonS Beck
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Feb 24, 2001·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·L C FigueiredoS L Salvador
May 24, 2001·Journal of Bacteriology·B J PasterF E Dewhirst
May 7, 2002·Journal of Periodontology·Ana Paula V ColomboMilton Uzeda
May 16, 2002·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Nov 26, 2002·Journal of Clinical Periodontology·Daniela C GrisiMárcio F M Grisi
Apr 24, 2003·Journal of Dental Research·P S KumarE J Leys
May 7, 2004·Clinical Implant Dentistry and Related Research·Asa LeonhardtUlf Lekholm
Aug 18, 2004·Brazilian Oral Research·Solange Alonso VerganiRosemary Adriana Chiérici Marcantonio
Oct 20, 2004·Oral Microbiology and Immunology·S S SocranskyJ M Goodson
Jan 26, 2006·Journal of Dental Research·Y LeeW J Loesche
Oct 25, 2008·Brazilian Oral Research·Daiane Cristina PeruzzoGetúlio da Rocha Nogueira-Filho

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Citations

Nov 7, 2012·Journal of Investigative and Clinical Dentistry·Thuy A V PhamYoko Kawaguchi
Jun 1, 2017·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Mervyn Sydney TurtonCharlene Wilma Joyce Africa
Jan 31, 2017·Colloids and Surfaces. B, Biointerfaces·Adaias O MatosValentim A R Barão
Jun 15, 2017·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Mahdi T KhadematolrasoulFaezeh Saadati

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