The ability of two different Vibrio spp. bacteriophages to infect Vibrio harveyi, Vibrio cholerae and Vibrio mimicus

Journal of Applied Microbiology
M PayneLeigh Owens

Abstract

To determine the host range of the Vibrio harveyi myovirus-like bacteriophage (VHML) and the cholera toxin conversion bacteriophage (CTX Phi) within a range of Vibrio cholerae and V. mimicus and V. harveyi, V. cholerae and V. mimicus isolates respectively. Three V. harveyi, eight V. cholerae and five V. mimicus isolates were incubated with VHML and CTX Phi. Polymerase chain reaction (PCR) was used to determine the presence of VHML and CTX Phi in infected isolates. We demonstrated that it was possible to infect one isolate of V. cholerae (isolate ACM #2773/ATCC #14035) with VHML. This isolate successfully incorporated VHML into its genome as evident by positive PCR amplification of the sequence coding part of the tail sheath of VHML. Attempts to infect all other V. cholerae and V. mimicus isolates with VHML were unsuccessful. Attempts to infect V. cholerae non-01, V. harveyi and V. mimicus isolates with CTX Phi were unsuccessful. Bacteriophage infection is limited by bacteriophage-exclusion systems operating within bacterial strains and these systems appear to be highly selective. One system may allow the co-existence of one bacteriophage while excluding another. VHML appears to have a narrow host range which may be related to a...Continue Reading

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Citations

Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Nancy Busico-Salcedo, Leigh Owens
Sep 9, 2016·Bacteriophage·Jens A HammerlStefan Hertwig
Mar 29, 2016·International Journal of Food Science·Sohana Al Sanjee, Md Ekramul Karim
May 26, 2015·Clinics in Laboratory Medicine·Romney M Humphries, Audrey N Schuetz
Dec 3, 2014·Applied and Environmental Microbiology·B A FroelichR T Noble
Jan 27, 2015·Applied and Environmental Microbiology·C D CruzG C Fletcher
Oct 1, 2020·Viruses·Santiago Hernández, Martha J Vives

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