The ability to organize sperm DNA into functional chromatin is acquired during meiotic maturation in murine oocytes

Developmental Biology
David W McLay, Hugh J Clarke

Abstract

Following fertilization of meiotically mature eggs, the chromatin of the sperm becomes biochemically and structurally remodeled within the egg cytoplasm. Despite the essential role of the paternal genome during embryogenesis, little is known of when the activities that regulate this chromatin remodeling appear during oogenesis. To determine whether these activities were acquired during meiotic maturation, we inseminated maturing oocytes of mice shortly after germinal vesicle breakdown. As previously shown, insemination at this stage did not activate the maturing oocytes, which became arrested at metaphase II. Immunofluorescent analysis revealed that at 1 hr postinsemination the sperm chromatin was dispersed and contained protamines but was devoid of core histones H2B and H3. At 4 hr postinsemination, both protamine and core histones were detectable on the sperm chromatin. By 8 hr postinsemination protamines were absent, and histones stained maximally. The appearance of immunoreactive histones was correlated with a morphological transition of the sperm chromatin from the dispersed to a condensed state, which suggests that the assembly of the histones reflected modification of the chromatin to a somatic-like state in which it was...Continue Reading

References

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Citations

Mar 21, 1998·Developmental Genetics·H J ClarkeO A Mohamed
Jul 8, 2008·Human Reproduction Update·Jason E Swain, Thomas B Pool
Jan 21, 2003·Biology of Reproduction·Daisuke KigamiHiroshi Imai
Jul 25, 1998·Genes & Development·N RougierE Viegas-Péquignot
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Yan XingHugh Clarke
Jun 30, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Luisa GioiaMauro Mattioli
Jan 8, 2008·Fertility and Sterility·Alexander WongPhilip J Chan
Jan 11, 2002·Developmental Biology·Fátima SantosWendy Dean
Oct 27, 2006·Cell Biochemistry and Function·Bing-zhi YuChen Feng
Aug 5, 2004·Molecular Reproduction and Development·Marcella SpinaciMauro Mattioli
Sep 8, 2005·Genesis : the Journal of Genetics and Development·Wei ShiReinald Fundele

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