The abnormal spindle protein is required for germ cell mitosis and oocyte differentiation during Drosophila oogenesis

Experimental Cell Research
Maria Giovanna RiparbelliGiuliano Callaini

Abstract

In this study, we present evidence that the asp function is required in oogenesis for germline cell divisions as well as for cyst polarity and oocyte differentiation. Consistent with previously described roles in spindle organization during Drosophila meiosis and mitosis, asp mutation leads to severe defects in spindle microtubule organization within the germarium. The mitotic spindles of the mutant cystocytes are composed by wavy microtubules and have abnormal poles that often lack gamma-tubulin. The fusome structure is also compromised. In the absence of asp function, the cystocyte divisions fail resulting in egg chamber with fewer than 16 germ cells. Moreover, the microtubule network within the developing germline cysts may assemble incorrectly in turn affecting the microtubule based transport of the specific determinants that is required during mid-oogenesis for the oocyte differentiation program.

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Citations

Sep 15, 2005·BMC Genomics·Zoë BirtleChris Ponting
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeremy N PulversWieland B Huttner
Dec 13, 2005·Current Opinion in Cell Biology·Jacquelyn Bond, C Geoffrey Woods
May 27, 2005·Current Opinion in Genetics & Development·Chris Ponting, Andrew P Jackson
Feb 28, 2018·Health Care for Women International·Sundus TariqKhalid Parvez Lone
Feb 6, 2021·Scientific Reports·Caitlin E McDonough-GoldsteinSteve Dorus

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