The absence of cruciform structures from pAO3 plasmid DNA in vivo

Journal of Biomolecular Structure & Dynamics
V I LyamichevS M Mirkin

Abstract

We extracted pAO3 plasmid DNA from E. coli cells, having "frozen" the transition between cruciform and double-helical conformations in DNA. The characteristic feature of the DNA isolation procedure is that all steps were carried out at temperature between 0 and 4 C and no phenol deproteinization was used, since it has been discovered that phenol destabilizes cruciform structures in pAO3 DNA. Two-dimensional gel electrophoresis has revealed no cruciform structures in the pAO3 DNA preparations obtained this way, although the superhelical density of DNA was sufficient for them. Cruciform structures are absent from intracellular pAO3 DNA at all growth stages of the bacterial culture: stationary and logarithmic, and under the induction of pAO3 DNA replication in chloramphenicol-treated cells.

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Citations

Jan 1, 1986·Progress in Biophysics and Molecular Biology·A Wada, A Suyama
Jun 23, 2004·The Journal of Biological Chemistry·Hiroki KurahashiTatsushi Toda
Nov 25, 1992·Nucleic Acids Research·A DaynS M Mirkin
May 31, 2013·Mikrobiologiia·O Iu LimanskaiaA P Limanskiĭ
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Jun 1, 1985·Journal of Biomolecular Structure & Dynamics·I PanyutinS Mirkin
May 1, 1993·Quarterly Reviews of Biophysics·D M Lilley, R M Clegg
Jan 15, 2020·Nature Communications·Jennifer A McKinneyKaren M Vasquez
Jan 1, 2013·Cytology and Genetics·O Yu LimanskayaA P Limanskii
Oct 1, 1985·Journal of Biomolecular Structure & Dynamics·V I LyamichevM D Frank-Kamenetskii
Jun 17, 1999·Journal of Molecular Biology·M Y Vologodskaia, A V Vologodskii

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