The absence of enhancer competition between Igf2 and H19 following transfer into differentiated cells.

Molecular and Cellular Biology
A L Webber, S M Tilghman

Abstract

H19 and Igf2 are reciprocally imprinted genes that lie 90 kb apart on mouse chromosome 7. The two genes are coexpressed during development, with the H19 gene expressed exclusively from the maternal chromosome and Igf2 from the paternal chromosome. It has been proposed that their reciprocal imprinting is governed by a competition between the genes for a common set of enhancers. The competition on the paternal chromosome is influenced by extensive allele-specific methylation of the H19 gene and its 5' flank, which acts to inhibit H19 transcription and thus indirectly leads to the activation of the Igf2 gene. In contrast, no allele-specific methylation has been detected on the maternal chromosome, and the basis for the preference for H19 transcription on that chromosome is unresolved. In this investigation, the mechanism controlling the silencing of the Igf2 gene on the maternal chromosome was explored by studying the transcriptional activity of a yeast artificial chromosome (YAC) containing Igf2 and H19 following transfer into differentiated tissue culture cells. Contrary to expectations, both H19 and Igf2 were expressed from a single integrated copy of the YAC. Furthermore, Igf2 expression appeared to be independent of the H19 l...Continue Reading

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Citations

Jun 24, 2000·European Journal of Biochemistry·F WilkinC Mamelin
Jul 18, 2018·American Journal of Physiology. Endocrinology and Metabolism·Mary Frances LopezJoel N Hirschhorn
Apr 17, 2020·Current Opinion in Genetics & Development·Daan Noordermeer, Robert Feil

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