The absence of functional bassoon at cone photoreceptor ribbon synapses affects signal transmission at Off cone bipolar cell contacts in mouse retina.

Acta Physiologica
Norbert BabaiAndreas Feigenspan

Abstract

Off cone bipolar cells of the mammalian retina connect to cone photoreceptor synaptic terminals via non-invaginating flat contacts at a considerable distance from the only established neurotransmitter release site so far, the synaptic ribbon. Diffusion from the ribbon synaptic active zone is considered the most likely mechanism for the neurotransmitter glutamate to reach postsynaptic receptors on the dendritic tips of Off cone bipolar cells. We used a mutant mouse with functionally impaired photoreceptor ribbon synapses to investigate the importance of intact ribbon synaptic active zones for signal transmission at Off cone bipolar cell contacts. Whole-cell patch-clamp recordings from Off cone bipolar cells in a horizontal slice preparation of wildtype (Bsnwt ) and mutant (BsnΔEx4/5 ) mouse retina were applied to investigate signal transmission between cone photoreceptors and Off cone bipolar cells. The distribution of postsynaptic glutamate receptors in Off cone bipolar cell dendrites was studied using multiplex immunocytochemistry. Tonic synaptic activity and evoked release were significantly reduced in mutant animals. Vesicle replenishment rates and the size of the readily releasable pool were likewise decreased. The precisel...Continue Reading

References

Nov 15, 1966·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J E Dowling, B B Boycott
Nov 20, 1983·The Journal of Comparative Neurology·J C Blanks, L V Johnson
May 26, 1999·The Journal of Comparative Neurology·K RichterE D Gundelfinger
Nov 17, 1999·The European Journal of Neuroscience·J H BrandstätterH Wässle
Mar 10, 2001·Cell and Tissue Research·J H Brandstätter, I Hack
Jul 12, 2001·The Journal of Comparative Neurology·S HaverkampH Wässle
Dec 23, 2003·The Journal of Comparative Neurology·Krishna K GhoshHeinz Wässle
Feb 20, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John E McRoryTerrance P Snutch
Feb 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher KushmerickHenrique von Gersdorff
Sep 11, 2007·Nature Neuroscience·Mitsuharu MidorikawaMasao Tachibana
Mar 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·David Zenisek
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chieko KoikeTakahisa Furukawa
Sep 2, 2011·Acta Physiologica·H Regus-Leidig, J H Brandstätter
Nov 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cole W GraydonHenrique von Gersdorff
May 3, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bart G BorghuisJonathan B Demb
May 30, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Theresa PuthusseryW Rowland Taylor
Jun 4, 2016·The European Journal of Neuroscience·Karlene M CorkWallace B Thoreson
Jun 24, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Norbert BabaiJohann Helmut Brandstätter
Nov 26, 2016·ELife·Christian BehrensPhilipp Berens
Mar 23, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Timur TsintsadzeStephen M Smith

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