The Absence of Sensory Axon Bifurcation Affects Nociception and Termination Fields of Afferents in the Spinal Cord

Frontiers in Molecular Neuroscience
Philip TrösterHannes Schmidt

Abstract

A cGMP signaling cascade composed of C-type natriuretic peptide, the guanylyl cyclase receptor Npr2 and cGMP-dependent protein kinase I (cGKI) controls the bifurcation of sensory axons upon entering the spinal cord during embryonic development. However, the impact of axon bifurcation on sensory processing in adulthood remains poorly understood. To investigate the functional consequences of impaired axon bifurcation during adult stages we generated conditional mouse mutants of Npr2 and cGKI (Npr2fl/fl;Wnt1 Cre and cGKIKO/fl;Wnt1 Cre ) that lack sensory axon bifurcation in the absence of additional phenotypes observed in the global knockout mice. Cholera toxin labeling in digits of the hind paw demonstrated an altered shape of sensory neuron termination fields in the spinal cord of conditional Npr2 mouse mutants. Behavioral testing of both sexes indicated that noxious heat sensation and nociception induced by chemical irritants are impaired in the mutants, whereas responses to cold sensation, mechanical stimulation, and motor coordination are not affected. Recordings from C-fiber nociceptors in the hind limb skin showed that Npr2 function was not required to maintain normal heat sensitivity of peripheral nociceptors. Thus, the ...Continue Reading

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Citations

Apr 24, 2020·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Alexandre DumoulinFritz G Rathjen
Apr 27, 2018·International Journal of Molecular Sciences·Alexandre DumoulinFritz G Rathjen
Oct 3, 2018·Frontiers in Neural Circuits·Steffen WolterLukas Rüttiger
Oct 16, 2018·Frontiers in Molecular Neuroscience·Alexandre DumoulinFritz G Rathjen
Jul 3, 2018·Frontiers in Cellular Neuroscience·Gohar Ter-AvetisyanFritz G Rathjen
Jan 20, 2020·Cellular and Molecular Neurobiology·Jonghoon KangYoung S Gwak
May 4, 2021·British Journal of Pharmacology·Hannes SchmidtAchim Schmidtko
Sep 17, 2021·Journal of Anatomy·Steven J MiddletonJohn M Dawes

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Methods Mentioned

BETA
PCR
genotyping
force measurements
transgenic

Key Resources (RRID) Mentioned

MGI
IMSR_JAX
MMRRC_015500
MMRRC_000364
AB_307210
AB_397865
AB_10077627
AB_2622233
AB_310180
AB_2315235

Software Mentioned

nTerim
3D
ZEN
MouseWalker
LabChart
nQuery
3D ImageJ Suite
ImageJ
ImageJ Suite
SPSS

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