The absence of the dna-dependent protein kinase catalytic subunit in mice results in anaphase bridges and in increased telomeric fusions with normal telomere length and G-strand overhang

Molecular and Cellular Biology
F A GoytisoloM A Blasco

Abstract

The major pathway in mammalian cells for repairing DNA double-strand breaks (DSB) is via nonhomologous end joining. Five components function in this pathway, of which three (Ku70, Ku80, and the DNA-dependent protein kinase catalytic subunit [DNA-PKcs]) constitute a complex termed DNA-dependent protein kinase (DNA-PK). Mammalian Ku proteins bind to DSB and recruit DNA-PKcs to the break. Interestingly, besides their role in DSB repair, Ku proteins bind to chromosome ends, or telomeres, protecting them from end-to-end fusions. Here we show that DNA-PKcs(-/-) cells display an increased frequency of spontaneous telomeric fusions and anaphase bridges. However, DNA-PKcs deficiency does not result in significant changes in telomere length or in deregulation of the G-strand overhang at the telomeres. Although less severe, this phenotype is reminiscent of the one recently described for Ku86-defective cells. Here we show that, besides DNA repair, a role for DNA-PKcs is to protect telomeres, which in turn are essential for chromosomal stability.

References

Apr 18, 1991·Nature·E H Blackburn
Jul 1, 1996·Nature Genetics·J A MetcalfeA M Taylor
May 1, 1996·Human Molecular Genetics·P M LansdorpH J Tanke
Oct 1, 1996·Molecular and Cellular Biology·J S DanskaC J Guidos
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·T BluntP A Jeggo
Oct 1, 1996·Trends in Biochemical Sciences·C Autexier, C W Greider
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·R ArakiM Abe
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M ZijlmansP M Lansdorp
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y GuF W Alt
Sep 18, 1997·The Journal of Experimental Medicine·H OuyangG C Li
Feb 26, 1998·Cell·B van SteenselT de Lange
May 23, 1998·Science·S GravelR J Wellinger
Jun 23, 1998·Current Biology : CB·T LarocheS M Gasser
Aug 14, 1998·Molecular Cell·G C LiC Cordon-Cardo
Mar 3, 1999·Genomics·P HandeP Lansdorp
Apr 24, 1999·Genes & Development·G C Smith, S P Jackson
May 25, 1999·Cell·J D GriffithT de Lange
Jul 20, 1999·The Journal of Biological Chemistry·A Bianchi, T de Lange
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·H VogelP Hasty
Sep 25, 1999·Nucleic Acids Research·M T Hemann, C W Greider
Sep 29, 1999·Genes & Development·M A BlascoJ Lingner
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H L HsuD J Chen
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M BaileyE H Goodwin
Feb 2, 2000·The EMBO Journal·E HerreraM A Blasco
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·D GisselssonN Mandahl
Nov 23, 2000·Genes & Development·H L HsuD J Chen

❮ Previous
Next ❯

Citations

Jan 5, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Michele BrunoriVincent Géli
Apr 22, 2005·Chromosoma·Predrag Slijepcevic, Suliman Al-Wahiby
Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Lea Harrington
Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Maria E Gallego, Charles I White
Jan 7, 2004·Biochemical and Biophysical Research Communications·Ayumi UrushibaraMasami Watanabe
Mar 11, 2004·DNA Repair·Susan M BaileyEdwin H Goodwin
Nov 13, 2002·Mutation Research·Larry H Thompson, David Schild
May 22, 2003·Cancer Letters·María A Blasco
May 22, 2003·Cancer Letters·Jan Karlseder
Nov 8, 2001·Current Opinion in Cell Biology·M Serrano, M A Blasco
Feb 8, 2003·Current Opinion in Genetics & Development·María A Blasco
May 28, 2003·DNA Repair·John Thacker, Małgorzata Z Zdzienicka
Oct 16, 2002·Nature Genetics·Marta García-CaoMaría A Blasco
Sep 2, 2005·Nature Reviews. Genetics·Maria A Blasco
Jan 25, 2003·European Journal of Biochemistry·Sabita N SaldanhaTrygve O Tollefsbol
Oct 30, 2008·The Journal of Biological Chemistry·Sarah S DurkinO John Semmes
Mar 5, 2003·The Journal of Experimental Medicine·Sean RooneyJoAnn Sekiguchi
Mar 5, 2008·The Journal of Experimental Medicine·Sonia FrancoFrederick W Alt
Nov 12, 2002·The EMBO Journal·Silvia EspejelMaría A Blasco
Jun 27, 2002·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Richard Marcotte, Eugenia Wang
Jun 28, 2002·Nucleic Acids Research·Chao WeiCarolyn M Price
Apr 13, 2005·Nucleic Acids Research·Nicholas S Y TingTara L Beattie
May 7, 2008·Molecular and Cellular Biology·Deyu GuanMichael B Mathews
Aug 12, 2009·Molecular and Cellular Biology·Claire L AttwoollJohn H J Petrini
Oct 5, 2011·Molecular and Cellular Biology·Raghavendra A ShamannaMichael B Mathews
Jun 8, 2002·Molecular and Cellular Biology·Anthony W I LoJohn P Murnane
Aug 5, 2003·Molecular and Cellular Biology·Isabel JacoMaría A Blasco
May 15, 2004·Molecular and Cellular Biology·Kyungjae MyungEric A Hendrickson
Jan 30, 2004·Molecular and Cellular Biology·Françoise DantzerValérie Schreiber
Jun 11, 2004·Annual Review of Biochemistry·Agata Smogorzewska, Titia de Lange
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Sheila A Stewart, Robert A Weinberg
Jul 11, 2006·Annual Review of Genetics·Karel RihaDorothy E Shippen
Feb 27, 2009·Cancer Research·Eli S WilliamsSusan M Bailey
Mar 23, 2011·Genome Integrity·Aurora Ruiz-HerreraElena Giulotto
Mar 23, 2005·Genetics·Larisa MelnikovaPavel Georgiev
Sep 11, 2008·Genetics·Mia Rochelle LowdenShawn Ahmed

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved