The accessory subunit of DNA polymerase gamma is essential for mitochondrial DNA maintenance and development in Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
Balaji IyengarAna Regina Campos

Abstract

DNA polymerase gamma, Pol gamma, is the key replicative enzyme in animal mitochondria. The Drosophila enzyme is a heterodimer comprising catalytic and accessory subunits of 125 kDa and 35 kDa, respectively. Both subunits have been cloned and characterized in a variety of model systems, and genetic mutants of the catalytic subunit were first identified in Drosophila, as chemically induced mutations that disrupt larval behavior (tamas). Mutations in the gene encoding the accessory subunit have not yet been described in any organism. Here, we report the consequences of null mutations upon mitochondrial DNA (mtDNA) replication and morphology, cell proliferation, and organismal viability. Mutations in the accessory subunit cause lethality during early pupation, concomitant with loss of mtDNA and mitochondrial mass, and reduced cell proliferation in the central nervous system. Surprisingly, the function of the central nervous system and muscle, as assessed in a locomotion assay, are only marginally affected. This finding is in contrast to our previous findings that disruption in the function of the catalytic subunit causes severe reduction in larval locomotion. We discuss our results in the context of current hypotheses for the funct...Continue Reading

References

Oct 3, 1999·The Journal of Biological Chemistry·Y Wang, L S Kaguni
Mar 25, 2000·Science·M D AdamsJ C Venter
Oct 19, 2000·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Y Goto
Oct 31, 2000·The Journal of Biological Chemistry·P J DiGregorioP H O'Farrell
Feb 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K F Ferri, G Kroemer
Sep 14, 2001·Biochemical and Biophysical Research Communications·K TakataK Sakaguchi
Dec 1, 1977·Wilhelm Roux's Archives of Developmental Biology·M Mandaravally Madhavan, Howard A Schneiderman

❮ Previous
Next ❯

Citations

Aug 28, 2003·Experimental Cell Research·Jessica MagnussonJan-Willem Taanman
Feb 9, 2006·Chemical Reviews·Maria A GraziewiczWilliam C Copeland
Mar 27, 2012·Nature Genetics·Eli A StahlRobert M Plenge
Apr 11, 2003·Molecular Biology of the Cell·Nuria GarridoJohannes N Spelbrink
May 11, 2011·Human Molecular Genetics·Matthew J YoungWilliam C Copeland
Jun 11, 2004·Annual Review of Biochemistry·Laurie S Kaguni
Jul 8, 2014·The International Journal of Biochemistry & Cell Biology·Shamik Banerjee, Balaji Chinthapalli
Dec 1, 2012·Human Molecular Genetics·Margaret M HumbleWilliam C Copeland
Oct 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Umut CaginJoseph M Bateman
Dec 27, 2011·Plant Science : an International Journal of Experimental Plant Biology·Shailesh KumarMinami Matsui
Aug 25, 2005·IUBMB Life·Rafael Garesse, Laurie S Kaguni
Mar 6, 2015·Genome Biology and Evolution·Marcos T OliveiraLaurie S Kaguni
Dec 8, 2017·Frontiers in Molecular Biosciences·Matthew J Young
Feb 5, 2009·Journal of Internal Medicine·A Spinazzola, M Zeviani
Sep 8, 2010·Developmental Disabilities Research Reviews·Shane L ReaMarni J Falk
Oct 6, 2005·Human Molecular Genetics·Peter R AndersonJohn P Phillips
Mar 1, 2008·The Journal of Biological Chemistry·Cristina AdánRafael Garesse
Jun 24, 2014·Molecular Systems Biology·Atsushi FukuohHoward T Jacobs
Jan 15, 2020·Fly·Steven J MarygoldMasamitsu Yamaguchi
Mar 6, 2021·ELife·Nikolay P KandulOmar S Akbari
Feb 4, 2010·Experimental Gerontology·Marcos T OliveiraLaurie S Kaguni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.