PMID: 1201007Jul 1, 1975Paper

The accumulation of aspartate in the presence of ethanol in rat liver

The Biochemical Journal
M Stubbs, H A Krebs

Abstract

1. Isolated hepatocytes were used to establish the reasons for the accumulation of aspartate, previously observed when the isolated rat liver was perfused with ethanol in the presence of alanine or ammonium lactate. 2. The isolated cells did not form aspartate when incubated with alanine and ethanol, but much aspartate was formed on incubation with ammonium lactate and ethanol. 3. Urea was the main nitrogenous product on incubation with alanine, in contrast with the perfused liver, where major quantities of NH4+ are also formed. When the formation of urea was nullified by the addition of urease, alanine plus ethanol caused aspartate formation, indicating that aspartate formation depends on the presence of critical concentrations of NH4+. 4. The accumulated aspartate was present in the cytosol. Ethanol halved the content of 2-oxoglutarate in the cytosol and more than trebled that of glutamate in the mitochondria. 5. The findings support the assumption that 2-oxoglutarate formed by the mitochondrial aspartate aminotransferase is not translocated to the cytosol in the presence of ethanol and NH4+, because it is rapidly converted into glutamate, the dehydrogenation of ethanol providing the required NADH. Aspartate, however, is tran...Continue Reading

Citations

Jul 12, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Tien-Chun WanRyoichi Sakata
Jan 1, 1987·Annals of the New York Academy of Sciences·M A RothschildS S Schreiber
May 1, 1981·European Journal of Biochemistry·P A Barton, N J Hoogenraad
Jun 1, 1981·Biochemical and Biophysical Research Communications·P M Stewart, M Walser
Jan 1, 1982·Western Journal of Nursing Research·R Ludeman
Aug 1, 1976·FEBS Letters·R Van Der Meer, J M Tager
Jul 11, 2019·Food Science & Nutrition·Thi Mong Tho NguyenYu-Wei Chang
Sep 1, 1979·Biochemical Pharmacology·M A Petit, I Barral-Alix
Oct 3, 1993·Biochimica Et Biophysica Acta·A Martín-RequeroR Parrilla
Nov 17, 1982·Biochimica Et Biophysica Acta·A J Meijer, G M Van Woerkom
Sep 14, 1987·Biochimica Et Biophysica Acta·R Hernández-MuñozV Chagoya de Sánchez
Jan 1, 1988·The International Journal of Biochemistry·G BánhegyiJ Mandl
Jan 1, 1986·The International Journal of Biochemistry·M WiechetekS Garwacki
Aug 31, 1982·Biochemical and Biophysical Research Communications·J E O'ConnorS Grisolía

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