The accuracy of serologic diagnosis of Helicobacter pylori infection in school-aged children of mixed ethnicity

Helicobacter
Y TindbergM Granström

Abstract

The present study evaluated two non-invasive diagnostic methods for H. pylori infection in children, i.e. an in-house ELISA using sonicated Campylobacter jejuni antigen for absorption of cross-reacting antibodies and an immunoblot kit (Helico Blot 2.0, Genelabs, Singapore). 13C -Urea breath test (13C-UBT) was used as reference Sera and questionnaires were collected from 695/858 (81%) Swedish school children with mixed ethnic backgrounds within a cross-sectional, community-based study. Of 133 children with an ELISA OD value of > or = 0.1, all were screened with immunoblot and 107 made a 13C-UBT. The negative controls were 34/37 children from three school classes with an ELISA OD value of < 0.1 and volunteering for a 13C-UBT. An adjusted cut-off level for the ELISA of OD value 0.22 resulted in a sensitivity of 97.8%, a specificity of 95.8% and a concordance index of 97.2%. The Helico Blot 2.0 had a sensitivity of 97.8%, a specificity of 93.8% and a concordance index of 96.5%. The best concordance was seen for the 26.5 kDa (98.6%), 30 kDa (95.7%) and 19.5 kDa (91.5%) antigens. The corresponding concordance index for CagA was 78%, for VacA 73.8% and for the 35kDa antigen 68.8%. A significant difference in the distribution of the 19...Continue Reading

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Citations

Aug 29, 2002·Helicobacter·Eleftheria Roma-Giannikou, Peter L Shcherbakov
Jul 2, 2005·Journal of Pediatric Gastroenterology and Nutrition·Ylva TindbergMarta Granström
Jan 6, 2010·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·J I KeenanF A Frizelle
Nov 9, 2005·Clinical and Diagnostic Laboratory Immunology·Masumi OkudaTeruko Nakazawa
Dec 10, 2003·Journal of Clinical Microbiology·Mårten KiviMarta Granström
Feb 10, 2004·Journal of Clinical Microbiology·Thi Thu Ha HoangMarta Granström
Nov 8, 2006·Digestive Diseases and Sciences·Catherine de Martel, Julie Parsonnet
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Apr 21, 2012·Helicobacter·Kazuki YamadaTeruko Nakazawa
Mar 30, 2017·British Journal of Cancer·E Christina M WennerströmCharles S Rabkin
Mar 31, 2017·Scandinavian Journal of Gastroenterology·G A AsgeirsdottirE S Björnsson
Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·Thi Thu Ha HoangMarta Granström

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