The acetylation state of human fetal hemoglobin modulates the strength of its subunit interactions: long-range effects and implications for histone interactions in the nucleosome

Biochemistry
Lois R Manning, J M Manning

Abstract

The source of the 70-fold increased tetramer strength of liganded fetal hemoglobin relative to that of adult hemoglobin between pH 6.0 and 7.5 reported earlier [Dumoulin et al. (1997) J. Biol. Chem. 272, 31326] has been identified as the N-terminal Gly residue of the gamma-chain, which is replaced by Val in adult hemoglobin. This was revealed by extending the study of the pH dependence of the tetramer-dimer equilibrium of these hemoglobins into the alkaline range as far as pH 9. From pH 7.5 to 9.0, the 70-fold difference in the association equilibrium constant between hemoglobins F and A lessened progressively. This behavior was attributed to the difference in the pK(a) 8.1 of Gly-1(gamma) compared to the pK(a) 7.1 value of Val-1(beta) of hemoglobins F and A, respectively. Evidence for this conclusion was obtained by demonstrating that natural hemoglobin F(1), which is specifically acetylated at Gly-1(gamma) and hence unable to be protonated, behaves like HbA and not HbF in its tetramer-dimer association properties over the pH range studied. An increased degree of protonation of the gamma-chain N-terminus of hemoglobin F from pH 9.0 to 8.0 is therefore suggested as responsible for its increased tetramer strength representing an...Continue Reading

Citations

Jan 1, 2003·Journal of Molecular Biology·Bogdan Polevoda, Fred Sherman
Dec 14, 2001·Protein Science : a Publication of the Protein Society·Takeshi YagamiJames M Manning
Aug 8, 2009·BMC Proceedings·Kristian K StarheimThomas Arnesen
Jun 3, 2005·Protein Science : a Publication of the Protein Society·Makoto AshiuchiJames M Manning
Jul 28, 2007·Protein Science : a Publication of the Protein Society·Lois R ManningJames M Manning
Jul 9, 2011·The FEBS Journal·Petra Van DammeKris Gevaert
Dec 2, 2011·The FEBS Journal·James M ManningLois R Manning
Jun 24, 2010·Protein Science : a Publication of the Protein Society·Lois R ManningJames M Manning
Jul 18, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shyang-Yun Pamela K ShiaoHector Pierantoni
Apr 10, 2016·Biochemical and Biophysical Research Communications·Daniel A RiccioTimothy J McMahon
Jul 4, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Andrew S DavisonNorman B Roberts
May 20, 2011·Analytical Chemistry·Félix O DupontChristiane Auray-Blais
Sep 22, 2006·Hemoglobin·Shyang-Yun Pamela K Shiao, Ching-Nan Ou
Jul 28, 2005·Rapid Communications in Mass Spectrometry : RCM·Patrick SturmMichel Ramonet
Oct 15, 2010·Free Radical Research·Janos KernerCharles L Hoppel
Dec 12, 2017·Protein Science : a Publication of the Protein Society·Lois R Manning, James M Manning
Apr 15, 2020·Analytical Biochemistry·Lois R Manning, James M Manning
Aug 24, 2021·Frontiers in Physiology·Barkha RaviGirdhar K Pandey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.