The Acinetobacter baumannii entA gene located outside the acinetobactin cluster is critical for siderophore production, iron acquisition and virulence.

PloS One
William F PenwellLuis A Actis

Abstract

Acinetobacter baumannii causes severe infections in compromised patients, who present an iron-limited environment that controls bacterial growth. This pathogen has responded to this restriction by expressing high-affinity iron acquisition systems including that mediated by the siderophore acinetobactin. Gene cloning, functional assays and biochemical tests showed that the A. baumannii genome contains a single functional copy of an entA ortholog. This gene, which is essential for the biosynthesis of the acinetobactin precursor 2,3-dihydroxybenzoic acid (DHBA), locates outside of the acinetobactin gene cluster, which otherwise harbors all genes needed for acinetobactin biosynthesis, export and transport. In silico analyses and genetic complementation tests showed that entA locates next to an entB ortholog, which codes for a putative protein that contains the isochorismatase lyase domain, which is needed for DHBA biosynthesis from isochorismic acid, but lacks the aryl carrier protein domain, which is needed for tethering activated DHBA and completion of siderophore biosynthesis. Thus, basF, which locates within the acinetobactin gene cluster, is the only fully functional entB ortholog present in ATCC 19606(T). The differences in a...Continue Reading

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Citations

Mar 8, 2013·Future Microbiology·Steven E Fiester, Luis A Actis
Jul 3, 2013·Infection and Immunity·Daniel L ZimblerLuis A Actis
Mar 13, 2017·BMC Microbiology·Karl A HassanBart A Eijkelkamp
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May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Aug 4, 2015·Chembiochem : a European Journal of Chemical Biology·William F PenwellLuis A Actis
Mar 30, 2017·Frontiers in Cellular and Infection Microbiology·Chang-Ro LeeSang Hee Lee
Aug 24, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hiroaki NakaMargo G Haygood
Aug 10, 2019·Frontiers in Microbiology·Maria S RamirezMarcelo E Tolmasky
Jul 28, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Phattaraporn KongthaiSutthirat Sitthisak
Feb 28, 2021·Scientific Reports·Jasmine MartinezMaria Soledad Ramirez
Oct 23, 2021·Frontiers in Microbiology·Kelly Conde-PérezMargarita Poza

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Datasets Mentioned

BETA
AB0057
AB307-294
AB307-0294
ACYR02000000.2
ACYQ00000000.2

Methods Mentioned

BETA
PCR

Software Mentioned

DNASTAR
ExPASy
BLAST
SAS
Sequencher

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