The actin-binding protein capulet genetically interacts with the microtubule motor kinesin to maintain neuronal dendrite homeostasis.

PloS One
Paul M B MedinaJay E Brenman

Abstract

Neurons require precise cytoskeletal regulation within neurites, containing microtubule tracks for cargo transport in axons and dendrites or within synapses containing organized actin. Due to the unique architecture and specialized function of neurons, neurons are particularly susceptible to perturbation of the cytoskeleton. Numerous actin-binding proteins help maintain proper cytoskeletal regulation. From a Drosophila forward genetic screen, we identified a mutation in capulet--encoding a conserved actin-binding protein--that causes abnormal aggregates of actin within dendrites. Through interaction studies, we demonstrate that simultaneous genetic inactivation of capulet and kinesin heavy chain, a microtubule motor protein, produces elongate cofilin-actin rods within dendrites but not axons. These rods resemble actin-rich structures induced in both mammalian neurodegenerative and Drosophila Alzheimer's models, but have not previously been identified by loss of function mutations in vivo. We further demonstrate that mitochondria, which are transported by Kinesin, have impaired distribution along dendrites in a capulet mutant. While Capulet and Cofilin may biochemically cooperate in certain circumstances, in neuronal dendrites t...Continue Reading

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Citations

Nov 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mei LiuPeter W Baas
Sep 29, 2011·BMC Neuroscience·April K MarroneHalyna R Shcherbata
Mar 13, 2015·Nature Protocols·Caroline MedioniFlorence Besse
Mar 21, 2020·Journal of Neurochemistry·Aleksandra TempesJacek Jaworski
Jul 26, 2018·The Journal of Cell Biology·Vanitha Nithianandam, Cheng-Ting Chien
Jul 6, 2012·Development·Julia NagelGaia Tavosanis

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Methods Mentioned

BETA
transgenic
PCR
dissection

Software Mentioned

LSM510
Adobe Illustrator
Capulet
Adobe Photoshop

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