The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells

Development
Alicia R ShieldsMargaret T Fuller

Abstract

Specialized microenvironments, or niches, provide signaling cues that regulate stem cell behavior. In the Drosophila testis, the JAK-STAT signaling pathway regulates germline stem cell (GSC) attachment to the apical hub and somatic cyst stem cell (CySC) identity. Here, we demonstrate that chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain GSCs, possibly facilitating localization or maintenance of E-cadherin to the GSC-hub cell interface. Germline specific overexpression of Adenomatous Polyposis Coli 2 (APC2) rescued GSC loss in chic hypomorphs, suggesting an additive role of APC2 and F-actin in maintaining the adherens junctions that anchor GSCs to the niche. In addition, loss of chic function in the soma resulted in failure of somatic cyst cells to maintain germ cell enclosure and overproliferation of transit-amplifying spermatogonia.

References

Mar 8, 1991·Science·P J Goldschmidt-ClermontT D Pollard
Dec 3, 1993·Cell·J A Theriot, T J Mitchison
Mar 21, 1998·Current Biology : CB·M Van DorenR Lehmann
Oct 5, 2001·Nature Cell Biology·B Baum, N Perrimon
Apr 11, 2002·Nature Cell Biology·Colin Jamora, Elaine Fuchs
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Jeffrey ChouAlan Wells
Sep 13, 2003·Science·Yukiko M YamashitaMargaret T Fuller
Aug 15, 2006·Current Opinion in Cell Biology·René-Marc MègeMireille Lambert
Jul 17, 2007·Current Biology : CB·Angshuman SarkarCordula Schulz
Sep 4, 2007·Experimental Cell Research·Mireille LambertRené-Marc Mège
Oct 18, 2007·Journal of Cell Science·Letizia CarramusaAlexander D Bershadsky
Jul 14, 2010·Nature Cell Biology·Judith L Leatherman, Stephen Dinardo
Feb 18, 2011·Journal of Cell Science·Swathi YadlapalliYukiko M Yamashita
Apr 6, 2011·PloS One·Catarina Gonçalves-PimentelAndreas Prokop
Nov 23, 2011·The Journal of Cell Biology·Sébastien NolaVania M M Braga
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jaclyn G Y Lim, Margaret T Fuller
Feb 19, 2013·Nature Cell Biology·Ridhdhi DesaiUlrich Tepass
Mar 14, 2013·Progress in Molecular Biology and Translational Science·Claudio Collinet, Thomas Lecuit
Apr 3, 2013·The Journal of Cell Biology·Soonjin HongSergey M Troyanovsky

❮ Previous
Next ❯

Citations

Jan 23, 2016·Nature Communications·Shree Ram SinghSteven X Hou
Mar 21, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Qianyi LuZhi Zheng
Apr 24, 2015·PLoS Pathogens·Irene L G NewtonKathy B Sheehan
May 27, 2017·The Journal of Biological Chemistry·Marion JoyPartha Roy
Mar 3, 2018·The Journal of Cell Biology·Dagan SegalBen-Zion Shilo
Mar 29, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun YuJie Fang
Jan 31, 2019·Development·Salvador C Herrera, Erika A Bach
Aug 23, 2017·The Journal of Biological Chemistry·Jing LiuRongjia Zhou
Jan 5, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
Feb 6, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
Oct 11, 2020·Mechanisms of Development·Antonio L Rockwell, Cintia F Hongay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.