The actin filament twist changes abruptly at boundaries between bare and cofilin-decorated segments

The Journal of Biological Chemistry
Andrew HuehnCharles V Sindelar

Abstract

Cofilin/ADF proteins are actin-remodeling proteins, essential for actin disassembly in various cellular processes, including cell division, intracellular transport, and motility. Cofilins bind actin filaments cooperatively and sever them preferentially at boundaries between bare and cofilin-decorated (cofilactin) segments. The cooperative binding to actin has been proposed to originate from conformational changes that propagate allosterically from clusters of bound cofilin to bare actin segments. Estimates of the lengths over which these cooperative conformational changes propagate vary dramatically, ranging from 2 to >100 subunits. Here, we present a general, structure-based method for detecting from cryo-EM micrographs small variations in filament geometry (i.e. twist) with single-subunit precision. How these variations correlate with regulatory protein occupancy reveals how far allosteric, conformational changes propagate along filaments. We used this method to determine the effects of cofilin on the actin filament twist. Our results indicate that cofilin-induced changes in filament twist propagate only 1-2 subunits from the boundary into the bare actin segment, independently of the boundary polarity (i.e. irrespective of wh...Continue Reading

References

Apr 25, 1995·Biochemistry·E M De La Cruz, T D Pollard
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Dec 28, 1999·Annual Review of Cell and Developmental Biology·J R Bamburg
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Oct 11, 2005·Journal of Molecular Biology·Ewa ProchniewiczEnrique M De la Cruz
Dec 27, 2005·Journal of Molecular Biology·Andrey A BobkovEmil Reisler
Jul 18, 2006·Journal of Molecular Biology·Wenxiang CaoEnrique M De La Cruz
Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
Oct 5, 2006·Molecular Cell·Ernesto Andrianantoandro, Thomas D Pollard
Dec 1, 2006·Journal of Molecular Biology·Dmitry PavlovEmil Reisler
Jan 23, 2009·Nature·Toshiro OdaAkihiro Narita
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jim PfaendtnerGregory A Voth
May 6, 2010·Biophysical Journal·Enrique M De La Cruz, David Sept
May 15, 2009·Biophysics Reviews·Enrique M De La Cruz
Oct 5, 2010·Methods in Enzymology·Edward H Egelman
Jul 5, 2011·Biophysical Journal·Brannon R McCulloughEnrique M De La Cruz
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vitold E GalkinEdward H Egelman
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Feb 12, 2013·FEBS Letters·W Austin ElamEnrique M De la Cruz
Aug 6, 2013·Biochemical and Biophysical Research Communications·W Austin ElamEnrique M De La Cruz
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kimihide HayakawaHitoshi Tatsumi
Dec 24, 2014·Structure·Vitold E GalkinEdward H Egelman
Apr 29, 2015·Current Biology : CB·Laurène GressinAlphée Michelot
May 10, 2015·The Journal of Biological Chemistry·Enrique M De La Cruz, Margaret L Gardel
Aug 10, 2016·Journal of Cell Science·Georgios Kanellos, Margaret C Frame
Feb 15, 2017·Journal of Structural Biology·Shaoda He, Sjors H W Scheres
Jun 22, 2017·Biophysical Journal·Anthony C SchrammEnrique M De La Cruz
Sep 25, 2017·The Journal of Biological Chemistry·W Austin ElamEnrique M De La Cruz
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ahmet MentesCharles V Sindelar

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Citations

Jan 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Andrew R HuehnCharles V Sindelar
Jul 30, 2020·The Journal of Biological Chemistry·Vivian W TangWilliam M Brieher
Sep 19, 2018·Nature Structural & Molecular Biology·Daniel V IwamotoDavid A Calderwood
Jul 28, 2019·Journal of Muscle Research and Cell Motility·Akihiro Narita
Jan 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hugo WiolandGuillaume Romet-Lemonne
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Roberto Dominguez
Nov 14, 2019·International Journal of Molecular Sciences·Vedud PurdeDmitri S Kudryashov
May 14, 2020·International Journal of Molecular Sciences·Francine ParkerMichelle Peckham
Apr 16, 2020·Physical Review. E·Adam S JermynMilo M Lin
Feb 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shoichiro Ono
Nov 27, 2020·Nature Communications·Andrew R HarrisDaniel A Fletcher
Jan 5, 2021·EMBO Reports·Hugo WiolandGuillaume Romet-Lemonne
Dec 15, 2019·Seminars in Cell & Developmental Biology·Felipe MerinoStefan Raunser
Dec 22, 2019·Seminars in Cell & Developmental Biology·Antoine Jegou, Guillaume Romet-Lemonne
Jan 29, 2021·The Journal of Biological Chemistry·Glen M HockyEnrique M De La Cruz
Feb 18, 2021·Journal of Chemical Theory and Computation·Sriramvignesh ManiGregory A Voth
Dec 22, 2020·Annual Review of Condensed Matter Physics·Shiladitya BanerjeeUlrich S Schwarz
Feb 2, 2021·Journal of Molecular Biology·Jeffrey P BibeauEnrique M De La Cruz
Jul 16, 2019·Biophysical Journal·Anthony C SchrammEnrique M De La Cruz
Nov 7, 2019·Chemical Reviews·Julien Robert-PaganinAnne Houdusse

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