The actin gene ACT1 is required for phagocytosis, motility, and cell separation of Tetrahymena thermophila

Eukaryotic Cell
N E WilliamsJ Frankel

Abstract

A previously identified Tetrahymena thermophila actin gene (C. G. Cupples and R. E. Pearlman, Proc. Natl. Acad. Sci. USA 83:5160-5164, 1986), here called ACT1, was disrupted by insertion of a neo3 cassette. Cells in which all expressed copies of this gene were disrupted exhibited intermittent and extremely slow motility and severely curtailed phagocytic uptake. Transformation of these cells with inducible genetic constructs that contained a normal ACT1 gene restored motility. Use of an epitope-tagged construct permitted visualization of Act1p in the isolated axonemes of these rescued cells. In ACT1Delta mutant cells, ultrastructural abnormalities of outer doublet microtubules were present in some of the axonemes. Nonetheless, these cells were still able to assemble cilia after deciliation. The nearly paralyzed ACT1Delta cells completed cleavage furrowing normally, but the presumptive daughter cells often failed to separate from one another and later became reintegrated. Clonal analysis revealed that the cell cycle length of the ACT1Delta cells was approximately double that of wild-type controls. Clones could nonetheless be maintained for up to 15 successive fissions, suggesting that the ACT1 gene is not essential for cell viabi...Continue Reading

References

Apr 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H KerskenH Plattner
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M HironoY Watanabe
Oct 1, 1987·The Journal of Cell Biology·J A Cooper
Jan 1, 1982·Methods in Cell Biology·L Van De WaterJ B Olmsted
Jul 1, 1995·The Journal of Eukaryotic Microbiology·N E WilliamsJ Frankel
May 5, 1997·The Journal of Cell Biology·T Kato-MinouraR Kamiya
Sep 30, 1999·Methods in Cell Biology·J Frankel
Sep 30, 1999·Methods in Cell Biology·K M Karrer
Sep 30, 1999·Methods in Cell Biology·D G Pennock
Sep 30, 1999·Methods in Cell Biology·K R Stuart, E S Cole
Oct 19, 2001·Biochemical and Biophysical Research Communications·H A Yanagisawa, R Kamiya
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuhua ShangMartin A Gorovsky
Dec 31, 2002·The Journal of Eukaryotic Microbiology·Robert V Zackroff, Linda A Hufnagel
Jan 17, 2003·Molecular Biology of the Cell·Charles GuerraPeter Satir
Jan 22, 2004·The Journal of Eukaryotic Microbiology·Roland E HoseinR H Gavin
Feb 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yifan LiuMartin A Gorovsky
Nov 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Roland KissmehlHelmut Plattner
Jun 10, 2005·Molecular Biology of the Cell·Alexander J Stemm-WolfMark Winey

❮ Previous
Next ❯

Citations

Nov 21, 2008·Molecular Biology of the Cell·Vidyalakshmi RajagopalanDavid J Asai
Jul 8, 2009·Eukaryotic Cell·Drashti DaveJacek Gaertig
Sep 6, 2007·The Journal of Cell Biology·Chandra L KilburnMark Winey
Mar 19, 2014·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Joseph S Briguglio, Aaron P Turkewitz
Dec 25, 2012·Surface and Interface Analysis : SIA·Ingela LanekoffAndrew G Ewing
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Feb 27, 2021·Science·Girish R MaliAndrew P Carter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Methods in Molecular Biology
Vidyalakshmi RajagopalanDavid E Wilkes
Advances in Experimental Medicine and Biology
Ling WuXiaoxia Li
Current Biology : CB
Kathleen Collins, M A Gorovsky
Biochemical and Biophysical Research Communications
Yi-Feng XiaJian-Guo Geng
© 2021 Meta ULC. All rights reserved