The actin network in the ciliary stalk of photoreceptors functions in the generation of new outer segment discs

The Journal of Comparative Neurology
I L HaleB Matsumoto

Abstract

Cytochalasin D (CD) interferes with the morphogenesis of outer segment disc membrane in photoreceptors. Disruption of either the actin network in the ciliary stalk, where membrane evagination is initiated, or the actin core of the calycal processes, whose position could define the disc perimeter, could be responsible. We have attempted to determine which of these local F-actin populations is involved in membrane morphogenesis and what step in the process is actin-dependent. Biocytin accumulation in nascent discs, detected by fluorescent avidin and laser scanning confocal microscopy (LSCM), provided a means of labeling abnormal discs and a measure of disc membrane addition. F-actin content and distribution were assessed using fluorescent phalloidin and LSCM. First, we examined the effects of a range of CD dosages (0.1, 1.0, or 10.0 microM) on rod photoreceptors in Xenopus laevis eyecup cultures. Ectopic outgrowth of discs, evaluated by LSCM and transmission electron microscopy (TEM), occurred at each concentration. Phalloidin labeling intensified in the ciliary stalk with increasing CD concentration, indicating F-actin aggregation. In contrast, it diminished in the calycal processes, indicating dispersal; TEM showed that calycal...Continue Reading

References

Aug 1, 1976·Experimental Eye Research·A M LatiesP Liebman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E WulfT Wieland
Feb 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L L Muller, T J Jacks
Nov 1, 1992·The American Journal of Physiology·N FrankiR M Hays
Apr 23, 1992·Nature·S A KuznetsovD G Weiss
Sep 1, 1991·The Journal of Cell Biology·L W JansonD L Taylor
Jul 1, 1991·Experimental Eye Research·M H Chaitin
Jan 1, 1991·Cell Motility and the Cytoskeleton·K Arikawa, D S Williams
Mar 1, 1990·Cell and Tissue Research·E Boisvieux-UlrichD Sandoz
Jan 1, 1985·Cell Motility·P A DetmersJ Condeelis
Dec 1, 1989·The Journal of Cell Biology·D K VaughanB Matsumoto
Jan 1, 1986·Biology of the Cell·B ChailleyD Sandoz
Jan 1, 1988·Biology of the Cell·D SandozG Bautista-Harris
Oct 1, 1987·The Journal of Cell Biology·J A Cooper
Mar 1, 1987·Experimental Eye Research·D K Vaughan, S K Fisher
May 1, 1974·The Journal of Cell Biology·A F MirandaS W Tanenbaum
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J T Wrenn, N K Wessells
Jan 15, 1971·Science·N K WessellsK Yamada
Oct 1, 1968·The Journal of Cell Biology·R W Young, B Droz
Sep 1, 1984·The Journal of Cell Biology·I VirtanenV P Lehto
Dec 1, 1984·The Journal of Cell Biology·L Marsh, P C Letourneau
Apr 1, 1980·The Journal of Comparative Neurology·R H SteinbergD H Anderson
Apr 1, 1983·Experimental Eye Research·J C Besharse, D A Dunis
Jun 1, 1981·The Journal of Cell Biology·P O'Connor, B Burnside
Nov 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R E GordonF Miller

❮ Previous
Next ❯

Citations

Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yun ZhaoTiansen Li
Apr 26, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Evan R BoitetAlecia K Gross
Jan 9, 2001·Journal of Cellular Physiology·R S LabowJ P Santerre
May 23, 2000·The Anatomical Record·M M Jablonski, C S Ervin
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Thomas BurgoyneClare E Futter
Dec 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·William J SpencerVadim Y Arshavsky
Aug 6, 2008·The Journal of Comparative Neurology·Nicole G GravagnaAngeles B Ribera
Nov 23, 2018·Biochemical Society Transactions·Roly Megaw, Toby W Hurd
Jul 31, 2004·The Journal of Biological Chemistry·Amir RattnerJeremy Nathans
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Laura Sánchez-BellverGemma Marfany

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.