The activated form of gelatinase B/matrix metalloproteinase-9 is associated with diabetic vitreous hemorrhage

Experimental Eye Research
Francis J DescampsAhmed M Abu El-Asrar

Abstract

We aimed to investigate the presence and activation status of matrix metalloproteinase (MMP)-2 and MMP-9 in vitreous samples from proliferative diabetic retinopathy (PDR) patients. Paired vitreous and serum samples were obtained from patients undergoing vitrectomy for the treatment of rhegmatogenous retinal detachment (RD) (65 patients) and PDR (67 patients). PDR patients were diagnosed for the presence of hemorrhage and/or patent new vessels. Quantitative assays were performed for vitreous protein content, MMP-2, MMP-9, tissue inhibitor of metalloproteinases-1 (TIMP-1) and hemoglobin. Qualitative evaluation of the MMP-2 and MMP-9 activation status was performed by zymography. Vitreous samples contained proMMP-2 but levels were unselectively related to total protein content. ProMMP-9 and activated MMP-9 levels were significantly increased in PDR patients (p<0.001 for both comparisons). In addition, TIMP-1 levels were significantly increased in PDR patients (p=0.004) and functionally inhibited activation of MMP-9 in vitreous samples. None of the parameters significantly differed between PDR patients with patent new vessels and those with inactive disease. However, activated MMP-9 levels in vitreous samples of PDR patients with h...Continue Reading

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Citations

Mar 1, 2012·Journal of Ocular Biology, Diseases, and Informatics·Ghulam Mohammad, Mohammad Mairaj Siddiquei
Feb 13, 2013·European Heart Journal·Josef FlammerStefano Taddei
May 15, 2013·Journal of Diabetes Research·Ahmed M Abu El-AsrarKarel Geboes
Aug 7, 2012·Acta Diabetologica·Ahmed M Abu El-AsrarGhislain Opdenakker
Feb 8, 2013·Acta Ophthalmologica·Ahmed M Abu El-AsrarKarel Geboes
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Jan 10, 2018·Frontiers in Immunology·Sonika RathiInderjeet Kaur
Aug 23, 2020·International Journal of Molecular Sciences·Luis García-OnrubiaSalvador Pastor-Idoate
Sep 24, 2020·Antioxidants·Ana Karen López-ContrerasAdolfo Daniel Rodríguez-Carrizalez
Jun 12, 2019·Cellular and Molecular Life Sciences : CMLS·Ghislain Opdenakker, Ahmed Abu El-Asrar

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