The activation domain of a hormone inducible HTLV-1 Rex protein determines colocalization with the nuclear pore

Experimental Cell Research
S RehbergerK Khazaie

Abstract

Human T-cell leukemia/lymphoma virus type 1 (HTLV-1) Rex is an essential regulatory protein that acts at the posttranscriptional level to promote expression of unspliced and singly spliced genes of the virus. Rex functions have been attributed to at least three separate domains of the protein determining nuclear/nucleolar accumulation and RNA binding (overlapping), multimerization, and nuclear export of Rex-responsive RNA. The steady-state intracellular localization of functional Rex molecules is mainly nucleolar. Fusions of wild-type Rex and the ligand binding domain of human estrogen receptor (ER) produced conditional molecules (ERRex and ERalaRex), which remained cytoplasmic in the absence of hormone and in response to hormone colocalized with the nuclear pore complex (NPC). These molecules induced in a hormone-dependent manner the expression of a Rex reporter plasmid and of the HTLV-1 Env protein and fusion of Env expressing cells. In contrast, activation domain mutants (ERRex delta and ERRexGly) translocated from the cytoplasm and acquired a diffuse nuclear localization. These mutants did not associate with the NPC and failed to show any of the expected Rex functions. Rex functions were perturbed by inactivating the RNA bi...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ChenH K Lyerly
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T J HopeT G Parslow
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Citations

Sep 13, 2005·Oncogene·Fatah Kashanchi, John N Brady
Dec 3, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Ihab Younis, Patrick L Green
Feb 2, 2016·Viruses·Jessica L MartinLouis M Mansky
Mar 21, 2002·The Journal of Biological Chemistry·Zhigang WengJackie Papkoff
Jul 5, 2019·Development, Growth & Differentiation·Regina Putri VirgiriniaAtsushi Suzuki

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