PMID: 9547266Jun 20, 1998Paper

The activation function 2 domain of hepatic nuclear factor 4 is regulated by a short C-terminal proline-rich repressor domain

Nucleic Acids Research
V P IyemereG G Brownlee

Abstract

Hepatic nuclear factor 4 (HNF4) is a transcription factor whose expression is crucial for mouse embryonic development, for liver-specific gene expression and for the prevention of one form of maturity-onset diabetes of the young. Its domain structure has been defined previously and is similar to other members of the nuclear receptor superfamily. A repressor domain has now been localised to a region of 14 amino acids (residues 428-441) near the C-terminus of HNF4 and is sufficient by itself to repress the activity of the activation function 2 (AF2) domain. Multiple mutations within this repressor domain enhance activity. Interestingly, this repressor domain shares homology with a repressor domain in the progesterone receptor. In a detailed mutagenesis study of the AF2 core, we demonstrate that L 366, which is conserved in the AF2 core between HNF4 and a number of orphan nuclear receptors, is essential for the full activity of the AF2 domain. Furthermore, a double mutation of E 363 and L 366 suggests that these residues might act in a cooperative manner.

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Citations

Feb 22, 2012·Cancer Metastasis Reviews·Luis A AparicioAngélica Figueroa
Oct 20, 2011·The Journal of Biological Chemistry·Thomas K FelderWolfgang Patsch
Jun 12, 2003·Nucleic Acids Research·Kay S Childs, Stephen Goodbourn
Apr 17, 2012·Journal of Cell Science·Wenli ZhouDavid C Hay
Feb 28, 2002·Molecular and Cellular Biology·Michael D RuseFrances M Sladek
Mar 9, 2005·Biochemical and Biophysical Research Communications·Arisa IharaIichiro Shimomura
Jul 18, 2015·Molecular and Cellular Endocrinology·Shivali R Patel, Debra F Skafar
Jul 9, 2010·Cancer Science·Eun-Yeung GongKeesook Lee
Jun 29, 2011·Biochemical and Biophysical Research Communications·Atsushi YokoyamaShigeaki Kato
Sep 19, 2018·Molecular and Cellular Biology·Shota SasakiYusuke Inoue
Mar 3, 2005·The Journal of Biological Chemistry·Anca D PetrescuAnn B Kier

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