The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry

Development
Ludmila FrancescattoRobert M Tombes

Abstract

Intracellular calcium ion (Ca(2+)) elevation on the left side of the mouse embryonic node or zebrafish Kupffer's vesicle (KV) is the earliest asymmetric molecular event that is functionally linked to lateral organ placement in these species. In this study, Ca(2+)/CaM-dependent protein kinase (CaMK-II) is identified as a necessary target of this Ca(2+) elevation in zebrafish embryos. CaMK-II is transiently activated in approximately four interconnected cells along the anterior left wall of the KV between the six- and 12-somite stages, which is coincident with known left-sided Ca(2+) elevations. Within these cells, activated CaMK-II is observed at the surface and in clusters, which appear at the base of some KV cilia. Although seven genes encode catalytically active CaMK-II in early zebrafish embryos, one of these genes also encodes a truncated inactive variant (alphaKAP) that can hetero-oligomerize with and target active enzyme to membranes. alphaKAP, beta2 CaMK-II and gamma1 CaMK-II antisense morpholino oligonucleotides, as well as KV-targeted dominant negative CaMK-II, randomize organ laterality and southpaw (spaw) expression in lateral plate mesoderm (LPM). Left-sided CaMK-II activation was most dependent on an intact KV, the...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·A S NayakM D Browning
Apr 5, 2002·The Biochemical Journal·Andy Hudmon, Howard Schulman
Jun 14, 2002·Current Biology : CB·Petra PennekampBernd Dworniczak
Jan 7, 2003·Genes & Development·Clifford J Tabin, Kyle J Vogan
May 29, 2003·The Journal of Cell Biology·Laird C SheldahlRandall T Moon
Dec 5, 2003·The Biochemical Journal·Roger J Colbran
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Oct 1, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Leslie C Griffith
Jul 5, 2005·Developmental Cell·Bhaskarjyoti SarmahSusan R Wente
Jul 29, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andy HudmonPaul De Koninck
Sep 28, 2005·Biochimica Et Biophysica Acta·Konstantin Lantsman, Robert M Tombes
Oct 6, 2005·Journal of Leukocyte Biology·Tara L HerrmannDavid J Kusner
Dec 24, 2005·Physiological Reviews·Michael Whitaker
Feb 8, 2006·European Journal of Medical Genetics·Hilde Peeters, Koen Devriendt
Apr 18, 2006·Cell·Nobutaka HirokawaSen Takeda
May 5, 2006·Development·Hidetaka Shiratori, Hiroshi Hamada
May 13, 2006·Brain Research·Charles A EasleyRobert M Tombes
Aug 25, 2006·Molecular Biology of the Cell·Heather O'LearyK Ulrich Bayer
Nov 15, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sarah C RothschildRobert M Tombes
Apr 20, 2007·Development·Xiaodong ShuJau-Nian Chen
May 18, 2007·Annual Review of Biochemistry·Ran ZalkAndrew R Marks
Jul 27, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Francoise GourroncMichael Rebagliati
Apr 16, 2008·Developmental Cell·Won-Suk Chung, Didier Y R Stainier
Apr 18, 2008·Journal of the American Society of Nephrology : JASN·Xiao FuAlbrecht G Kramer-Zucker
May 9, 2008·Cellular and Molecular Life Sciences : CMLS·M T Swulius, M N Waxham

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Citations

Aug 18, 2012·Disease Models & Mechanisms·Laura N VandenbergMichael Levin
May 6, 2011·Cellular and Molecular Life Sciences : CMLS·Yoichi Kato
Apr 25, 2012·Cellular and Molecular Life Sciences : CMLS·Takaaki Matsui, Yasumasa Bessho
Mar 30, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhu SongChengtian Zhao
Jan 31, 2012·Current Opinion in Cell Biology·Nobutaka HirokawaYasushi Okada
Jan 28, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sizhou HuangLingfei Luo
May 7, 2015·Biology of the Cell·Teresa Casar TenaMelanie Philipp
Jul 23, 2014·Developmental Neurobiology·Birbickram RoyDeclan W Ali
Feb 1, 2014·Bioarchitecture·Jeffrey D Amack
Feb 8, 2014·Communicative & Integrative Biology·Laura N VandenbergMichael Levin
Jul 30, 2016·Frontiers in Bioengineering and Biotechnology·Romina D'AurizioMarco Pellegrini
Nov 9, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rebecca D Burdine, Daniel T Grimes
Jun 15, 2017·ELife·Rita R FerreiraJulien Vermot
Apr 11, 2018·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sarah C RothschildRobert M Tombes
Jun 11, 2017·Cold Spring Harbor Perspectives in Biology·Joseph ZinskiMary C Mullins
Aug 4, 2020·Science Advances·Katsutoshi MizunoHiroshi Hamada
Mar 9, 2017·Frontiers in Cell and Developmental Biology·Samantha J EnglandKatharine E Lewis
Apr 4, 2015·G3 : Genes - Genomes - Genetics·Tania ManchenkovFarhad B Imam
Aug 23, 2012·Development·Tetsuya Nakamura, Hiroshi Hamada
Jul 15, 2011·Development·Sarah C RothschildRobert M Tombes

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