The Activation of Phytophthora Effector Avr3b by Plant Cyclophilin is Required for the Nudix Hydrolase Activity of Avr3b

PLoS Pathogens
Guanghui KongYuanchao Wang

Abstract

Plant pathogens secrete an arsenal of effector proteins to impair host immunity. Some effectors possess enzymatic activities that can modify their host targets. Previously, we demonstrated that a Phytophthora sojae RXLR effector Avr3b acts as a Nudix hydrolase when expressed in planta; and this enzymatic activity is required for full virulence of P. sojae strain P6497 in soybean (Glycine max). Interestingly, recombinant Avr3b produced by E. coli does not have the hydrolase activity unless it was incubated with plant protein extracts. Here, we report the activation of Avr3b by a prolyl-peptidyl isomerase (PPIase), cyclophilin, in plant cells. Avr3b directly interacts with soybean cyclophilin GmCYP1, which activates the hydrolase activity of Avr3b in a PPIase activity-dependent manner. Avr3b contains a putative Glycine-Proline (GP) motif; which is known to confer cyclophilin-binding in other protein substrates. Substitution of the Proline (P132) in the putative GP motif impaired the interaction of Avr3b with GmCYP1; as a result, the mutant Avr3bP132A can no longer be activated by GmCYP1, and is also unable to promote Phytophthora infection. Avr3b elicits hypersensitive response (HR) in soybean cultivars producing the resistance p...Continue Reading

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Citations

Jul 1, 2016·Annual Review of Phytopathology·Steven A WhithamTarek Hewezi
Aug 12, 2016·PLoS Pathogens·Suomeng Dong, Yuanchao Wang
Jan 12, 2017·Scientific Reports·Hemanta Raj MainaliSangeeta Dhaubhadel
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Dec 14, 2017·Nature Communications·Jie HuangSuomeng Dong
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Jan 1, 2020·Pathogens·Yan ZhangRuiwu Yang
Dec 1, 2020·Microbiology·Elzbieta Kraszewska, Joanna Drabinska
Feb 16, 2021·Frontiers in Plant Science·Tengfei LiuShuzhen Zhang

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Datasets Mentioned

BETA
PRX146-36

Methods Mentioned

BETA
two-hybrid
Y2H
co-immunoprecipitation
protein folding
PCR
two hybrid
pull-down
pull down

Software Mentioned

BLAST
ClustalW

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