The activation state of macrophages alters their ability to suppress preadipocyte apoptosis

The Journal of Endocrinology
André S D MolgatAlexander Sorisky

Abstract

Adipose tissue contains macrophages whose state of activation is regulated as obesity develops. Macrophage-secreted factors influence critical processes involved in adipose tissue homeostasis, including preadipocyte proliferation and differentiation into adipocytes. Macrophage-conditioned medium (MacCM) from J774A.1 macrophages protects 3T3-L1 preadipocytes from apoptosis through platelet-derived growth factor (PDGF) signaling. Here, we investigated the effect of macrophage activation on MacCM-dependent preadipocyte survival. MacCM was prepared following activation of either J774A.1 macrophages with lipopolysaccharide (LPS) or human primary monocyte-derived macrophages (MD-macrophages) with LPS or interleukin 4 (IL4). 3T3-L1 and human primary preadipocytes were induced to undergo apoptosis in MacCM, and apoptosis was quantified by cell enumeration or Hoechst nuclear staining. Preadipocyte PDGF signaling was assessed by immunoblot analysis of phosphorylated PDGF receptor, Akt, and ERK1/2. Pro-inflammatory activation of J774A.1 macrophages with LPS inhibited the pro-survival activity of MacCM on 3T3-L1 preadipocytes, despite intact PDGF signaling. Upregulation of macrophage tumor necrosis factor a (TNFα) expression occurred in re...Continue Reading

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Citations

Mar 7, 2013·The Journal of Endocrinology·Annemarie GagnonAlexander Sorisky
Jan 15, 2013·Experimental Cell Research·Wei-jun PangGong-she Yang
May 10, 2016·Canadian Journal of Diabetes·Vian PeshdaryAlexander Sorisky
Dec 3, 2016·PloS One·Ana Cristina Resende CamargosAna Cristina Rodrigues Lacerda
Nov 22, 2017·Stem Cells International·Fernando LizcanoAstrid Torrado

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