Feb 1, 1976

The activator of human cerebroside sulphatase. Activating effect on the acidic forms of the sulphatases from invertebrates

Hoppe-Seyler's Zeitschrift für physiologische Chemie
W MrazH Jatzkewitz

Abstract

1) Acidic forms of the sulphatase were partially purified from the following invertebrate species: Tethya aurantium (Porifera), Patella vulgata (mollusca), Maja squinado (Arthropoda), Marthasterias glacialis (Echinodermata) and Microcosmus sulcatus (Tunicata). Enzyme preparations thus obtained cleaved cerebroside sulphates (sulphatides) only in the presence of either specific detergents (e.g. taurodeoxycholate) or an activator protein isolated from human liver. This corresponds to the findings on purified sulphatase A of human origin. 2) At low concentrations, the activating effect was proportional to the amount of activator protein applied; at higher concentrations, proportionality was obtained only in some cases. On a molar basis, less of the activator protein was required to achieve the same activation as taurodeoxycholate. At optimum concentrations of the detergent however, the activation was much higher. 3) The enzyme specificity of the activator and some evolutionary implications are discussed.

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Citations

Mentioned in this Paper

Taurocholic Acid, Monolithium Salt
Beta-N-Acetylglucosaminidase
Brachiopoda
Sulfoglycosphingolipids
Mollusca
Cleaved Cell
Cerebrosides
Enzyme agent
Enzyme preparations, digestives
Molar Tooth

About this Paper

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