The active human gut microbiota differs from the total microbiota

PloS One
Francesc Peris-BondiaGiuseppe D'Auria

Abstract

The human gut microbiota is considered one of the most fascinating reservoirs of microbial diversity hosting between 400 to 1000 bacterial species distributed among nine phyla with Firmicutes, Bacteroidetes and Actinobacteria representing around 75% of the diversity. One of the most intriguing issues relates to understanding which microbial groups are active players in the maintenance of the microbiota homeostasis.Here, we describe the diversity of active microbial fractions compared with the whole community from raw human fecal samples. We studied four healthy volunteers by 16S rDNA gene pyrosequencing. The fractions were obtained by cell sorting based on bacterial RNA concentration. Bacterial families were observed to appear or disappear on applying a cell sorting method in which flow cytometry was used to evaluate the active cells by pyronin-Y staining of RNA. This method was able to detect active bacteria, indicating that the active players differed from that observed in raw fecal material. Generally, observations showed that in the active fractions, the number of reads related to Bacteroidetes decreased whereas several families from Clostridiales (Firmicutes) were more highly represented. Moreover, a huge number of familie...Continue Reading

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Methods Mentioned

BETA
PCR
biopsy
flow cytometry

Related Concepts

Feces
Flow Cytometry
RNA, Bacterial
RNA, Ribosomal, 16S
Bacteriocin Typing
Nested Polymerase Chain Reaction
Fluorescent in Situ Hybridization
Sequence Determinations, DNA
Gastrointestinal Tract Structure
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